NINJ2基因rs11833579和rs12425791位点多态性与中国汉族人群缺血性脑卒中相关性的Meta分析

Association between single-nucleotide polymorphisms of rs11833579/rs12425791 in Ninjurin 2 gene and ischemic stroke in Chinese Han population： evidence from a Meta-analysis

目的评价神经损伤诱导蛋白2（Ⅳ刷应）基因rs11833579和rs12425791位点多态性与中国汉族人群缺血性脑卒中（IS）的相关性。方法计算机检索PubMed、Embase、TheCochraneLibrary、中国学术文献总库（CNKI）、中国生物医学文献数据库（CBM）、维普（VIP）和万方数据库，搜集关于NINJ2基因rs11833579和rs12425791位点多态性与中国汉族人群IS发病风险相关性的病例对照研究，检索时限均自建库至2016年8月。采用Stata12．0软件对各研究结果进行异质性检验、数据合并及发表偏倚评估，应用Meta分析揭示rs11833579和rs12425791位点多态性与中国汉族人群IS的关系。结果rs11833579位点共纳入11项研究，包括病例组6125例，对照组6067例。rs12425791位点共纳人11项研究，包括病例组5989例，对照组6145例。Meta分析结果显示：NINJ2基因rs12425791位点的小等位基因（A）将显著增加罹患中国汉族人群大动脉粥样硬化（LAA）型IS的风险[AA／（GA＋GG）：OR=1．594，95％CI=1．155～2．201，P=0．050；AA／GG：OR=1．731，95％CI=1．240～2．416，P=0．001]。rs11833579位点多态性与中国汉族人群IS无显著相关性。Egger’和Begg’检验结果提示无发表偏倚。敏感性分析显示单个研究的剔除不影响合并结果。结论基于当前证据显示NINJ2基因rs12425791位点多态性与中国汉族人群LAA型IS的发病具有显著相关性，未发现rs11833579位点基因多态性与中国汉族人群脑卒中具有相关性。

Objective To study the association between single-nucleotide polymorphisms （SNPs） ofrs11833579/rs12425791 in Ninjurin 2 （NINJ2） gene and risk ofischemic stroke in Chinese Han population. Methods PubMed, CBM, The Cochrane Library, CNKI, CBM, VIP and Wanfang Data were searched from inception to August 2016 to collect case-control studies about SNPs of rs11833579/rs12425791 in NINJ2 gene and risk of ischemic stroke in Chinese Han population. Then, Meta-analysis was performed using Stata 12.0 software to extract the data and assess the risk of bias of included studies. This Meta analysis revealed the association between SNPs of rsl1833579/rs12425791 and ischemic stroke. Results Eleven independent publications, including 6125 cases and 6067 controls for rs1183379, and 5989 cases and 6145 controls for rs12425791, were chosen. Overall, large artery atherosclerotic ischemic stroke risk was associated with rsl 1833579 A allele when all studies were pooled into the Meta-analysis [AA/（GA＋GG）： OR=1.594, 95%CI： 1.155-2.201, P=-0.05; AA/GG： OR= 1.731, 95%CI： 1.240-2.416, P=-0.001]. However, rs12425791 was not associated with increased stroke risk in any genetic models. No publication bias was found in our Meta-analysis by Egger＇s and Begg＇s test. Sensitivity analysis confirmed the stability of results. Conclusion The rs11833579 SNPs may be a susceptibility marker of large artery atherosclerotic of ischemic stroke in Chinese Han population and further studies are warranted to verify this association.