长链非编码RNASNHG8在EB病毒相关胃癌中的表达及其临床意义

Expression of long non-coding RNA SNHG8 in Epstein-Barr virus-related gastric cancer and clinical outcome

目的探讨长链非编码RNASNHG8在EB病毒相关胃癌中的表达及其临床意义。方法收集2012年6月至2015年4月于福建省肿瘤医院进行外科手术的93例胃癌病例（癌组织和癌旁组织）；采用EB病毒编码的小RNA（EBER）原位杂交检测EB病毒的表达；即时荧光定量PCR验证上述组织中SNHG8的表达，并分析其与临床病理特征的关系。结果胃癌细胞核阳性的病例定义为EB病毒相关胃癌，共41例；93例胃癌患者中，SNHG8在肿瘤组织的表达比癌旁组织上调14倍（P=0．001）；同时EB病毒相关胃癌组织SNHG8表达比EB病毒阴性胃癌组织表达上调25倍（P=0．027）。EB病毒相关胃癌肿瘤组织中SNHG8的表达水平与TNM分期显著相关（P〈0．05）。结论SNHG8可能作为一个原癌基因，参与胃癌的发生发展；EB病毒感染胃黏膜可能促进SNHG8的表达，为EB病毒相关胃癌的致病机制研究提供新思路。

Objective To investigate the expression of long non-coding RNA （lncRNA） SNHG8 in EB virus related gastric cancer and their correlation prognosis. Methods The expression of SNHG8 in 93 gastric cancers and 93 cancer-free controls, matched by age and sex ,were determined by real-time PCR. EB virus expression was detected by EBER in situ hybridization. Results Forty-one gastric cancers were EB virus associated. For all gastric cancers, SNHG8 expression was 14 times higher （ P = 0. 001 ） than that in non-cancer controls ;in the EB virus related gastric cancers, SNHG8 expression was increased 25 times （ P 〈 0. 05 ） over EB virus negative gastric cancers. SNHG8 expression level was also significantly associated with TNM staging （ P 〈 0. 05 ）. Conclusions SNHG8 may act as a proto-oneogene, participating in gastric carcinogenesis. EB virus infection of gastric mueosa may promote SNHG8 expression.