缺氧诱导因子1α、葡萄糖转运蛋白1和乳酸脱氢酶5在结直肠癌的表达及其意义

Expression of hypoxia-inducible factor 1α, glucose transporter 1 and lactate dehydrogenase 5 in colorectal cancer and clinicopathologic significance

目的探讨缺氧诱导因子1α（HIF-1α）、葡萄糖转运蛋白1（GLUT-1）及乳酸脱氢酶5（LDH-5）在结直肠癌中的表达及其临床病理意义。方法采用免疫组织化学sP法检测142例结直肠癌组织及其癌旁组织中HIF-1α、GLUT-1及LDH-5蛋白的表达。结果HIF-1α、GLUT-1及LDH．5在结直肠癌组织的表达率分别为78．2％（111／142）、75．4％（107／142）及68．3％（97／142），与癌旁组织的表达率（14．8％，21／142；11．3％，16／142；7．O％，10／142）比较，其差异均具有统计学意义（P〈0．01）。结直肠癌组织中HIF-1α、GLUT-1及LDH-5的阳性表达率与淋巴结转移、肿瘤分化程度和病理分期有相关性（均P〈0．05）；结直肠癌中HIF-1α、GLUT-1和LDH-5的表达两两之间具有关联性，并且表达呈正相关（P〈0．05）。结论联合检测HIF-1α、GLUT-1及LDH-5在结直肠癌的表达，可作为评估结直肠癌的发展、转移及预后重要指标。

Objective To investigate the expression and clinicopathological significance of hypoxia- inducible factor 1 alpha （HIF-1α）, glucose transporter 1 （GLUT-1） and lactate dehydrogenase（LDH）-5 in colorectal cancer. Methods The expression levels of HIF-1α, GLUT-1 and LDH-5 were detected by immunohistochemical staining in 142 specimens of human carcinoma in comparison with adjacent normal tissues. Results The expression levels of HIF-1α（78.2% , 111/142） , GLUT-1 （75.4% , 107/142） and LDH-5 （68.3% , 97/142 ） were higher in tumor tissues than in adjacent normal tissues （ 14. 8% , 21/142 ; 11.3 % , 16/142 ; 7. 0 % , 10/142 ; P 〈 0. 01 for all three proteins ）, and such over-expression was significantly associated with lymphovascular invasion, tumor grade and pathological stages （ all P 〈 0. 01 ）. Additional studies showed that HIF-1α, GLUT-1 and LDH-5 were positively associated with each other（ r 〈 0. 3, P 〈 0.05 for all three proteins ）. Conclusion The data suggest that HIF-1α, GLUT-1 and LDH-5 expression may serve as prognostic indicators for colorectal cancer patients.