HPLC法测定麻黄鱼腥草散中黄芩苷的含量

Determination of baicalin in Mahuang Yuxingcao powder by HPLC

建立麻黄鱼腥草散中黄芩苷含量的测定方法。色谱条件:Waters XBrige TM C18(4.6 mm×150 mm,5μm);流动相:甲醇-0.2%磷酸溶液,梯度洗脱;流速:1.0 m L/min;柱温:30℃;检测波长:276 nm。黄芩苷的线性范围为0.079 8~0.797 5μg,R2=1.000 0,平均回收率为94.3%(RSD=0.8%)。本方法快速、简便、准确,可有效测定麻黄鱼腥草散中黄芩苷的含量。

To establish an HPLC method for determination of baicalin in Mahuang Yuxingcao powder.The baicalin was analyzed on an XBrigeTMC18column（4.6 mm ×250 mm,5 μm） using ultrapure methanol-0.2% phosphoric acid solution as the mobile phase.The flow rate was 1.0 m L/min.The column temperature was 30 ℃.The detection wavelength was set at 276 nm.The calibration curve was linear in the range of 0.079 8~0.797 5 μg（R2=1.000 0） for baicalin.The recovery of baicalin was 94.3% with RSD of 0.8%.This method is rapid,simple and accurate,which can be used for the determination of baicalin in Mahuang Yuxingcao powder.