秃鹫黏质沙雷氏菌和大肠杆菌的分离、鉴定及药敏试验

Separation,identification and drug sensitivity test of Serratia marcescens and Escherichia coli in vulture

采集死亡秃鹫的心脏、肝脏病灶进行细菌分离鉴定、生化试验,其病原菌为沙雷氏菌和大肠杆菌;经药敏试验,大肠杆菌对环丙沙星、庆大霉素、氯霉素极度敏感,对头孢哌酮、头孢他啶、头孢吡肟高度敏感,对恩诺沙星中度敏感,对新生霉素、多黏菌素B、四环素低度敏感;黏质沙雷氏菌对头孢哌酮、头孢他啶、恩诺沙星高度敏感,对头孢吡肟、环丙沙星中度敏感,对庆大霉素、氯霉素、新生霉素、多黏菌素B、四环素低度敏感。采用对黏质沙雷氏菌和大肠杆菌均属高度敏感的药物头孢哌酮治疗患病秃鹫,陆续痊愈。

Kunming zoo vultures appeared depressed,head shook,green outflow mucinous fluid in the mouth and other symptoms.By RT-PCR detection,Newcastle disease and avian influenza infection were excluded.Samples were collected from dead vultures in heart and liver,Serratia spp.and Escherichia coli were found;Drug susceptibility test showed that E.coli was extremely sensitive to ciprofloxacin,gentamicin and chloramphenicol,sensitive to cefoperazone,ceftazidime and cefepime,moderate sensitive to enrofloxacin,and muting sensitivity to novobiocin,polymyxin B and tetracycline;Serratieae was sensitive to cefoperazone,ceftazidime,and enrofloxacin,moderate sensitive to cefepime and ciprofloxacin,and muting sensitivity to gentamicin,chloramphenicol,neomycin,polymyxin B and tetracycline sensitive.After treated with cefoperazone,which was sensitive to S.bacteria and E.coli,the vultures recovered gradually.