摘要
采用正交试验方法研究愈骨葆方的最佳水提取工艺。通过血清药理学,以提取物对成骨细胞碱性磷酸酶(ALP)的活性的影响作为考察指标,采用L_9(3~4)正交表设计试验,对加水量(A)、加热时间(B)、浸泡时间(C)及煎煮次数(D)4个因素进行考察,分析最佳提取条件,并验证该工艺的有效性。通过该实验得出愈骨葆方最佳提取工艺:8倍加水量,加热时间60min,浸泡时间30 min,煎煮1次。按此工艺获得的愈骨葆方含药血清能显著地促进成骨相关基因的转录。该实验建立了愈骨葆方的最佳提取工艺,为该复方中药的开发利用提供了试验依据。
To determine the optimum aqueous extract protocol for Yugubao traditional Chinese medicines formula by using orthogonal experiment design. Through serum pharmacology research,L9（34） orthogonal design with single factor investigation was used to optimize the aqueous extract protocol for Yugubao formula. The effect of water extraction on activity of alkaline phosphatase（ ALP） in osteoblast was referred as the evaluation index for investigating four factors： water consumption（ A）,heating time（ B）,soaking time（ C）,and number of decocting（D）,analyzing the optimum extraction conditions,and verifying the effectiveness of this process. The optimum aqueous extract protocol for Yugubao was as follows： adding 8 times water into Chinese medical materials,heating for 60 min,soaking for 30 min,and decocting for 1 time. The drug serum of this aqueous extract of Yugubao could significantly up-regulate the osteogenic genes expression. The optimum aqueous extract protocol for Yugubao formula was established in this experiment,providing evidence for the development and utilization of Yugubao traditional Chinese medicines formula.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2017年第2期280-284,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金青年基金项目(81203003)
