龙州山橙的生物碱类成分研究

Studies on alkaloids from Melodinus moraei

对龙州山橙的生物碱类成分进行研究。采用硅胶柱色谱、反相柱色谱、高效制备液相等方法,对龙州山橙的总生物碱进行分离和纯化,并通过1H,13C-NMR,MS等波谱方法鉴定化合物的结构。该研究共分离得到7个生物碱类化合物,分别为scandine(1),tabersonine(2),melodinine N(3),melodinine P(4),melodinine T(5),19-epimeloscandonine(6),16-hydroxy-19 S-vindolinine(7)。化合物均1~7为首次从该植物中分离得到。采用MTT法对分离得到的化合物进行体外抗肿瘤活性评价,发现化合物1~7均表现出一定的体外细胞毒活性。

To study the alkaloids from Melodinus moraei,seven compounds were separated and purified by column chromatography over silica gel,reverse phase silica gel and preparative HPLC. Their chemical structures were elucidated by MS and spectral data（1HNMR,13C-NMR） as scandine（ 1）,tabersonine（ 2）,melodinine N（ 3）,melodinine P（ 4）,melodinine T（ 5）,19-epimeloscandonine（6）,16-hydroxy-19S-vindolinine（7）. Compounds 1-7 were isolated from M. moraei for the first time. The bioassays showed all tested samples displayed antitumor activity against the cell lines such as A549,PC-3,HGC-27 and HL-60.