奶牛黄体囊肿的蛋白质组学研究

Proteomics of Corpus Luteum Cysts in Dairy Cows

为了研究奶牛黄体囊肿的发病机理及发病时的生物学标记物,利用蛋白质组学经典的双向电泳(2-DE)技术,检测和分析奶牛黄体囊肿组织和正常黄体组织间的差异蛋白,通过考马斯亮蓝染色后用PDQuest8.0软件检测并分析差异蛋白点。经MALDI TOF/TOF-MS/MS串联质谱鉴定,共发现6种差异表达的蛋白,分别为:α-1-酸性糖蛋白(AGP),胶质纤维酸性蛋白(GFAP),核磷蛋白(NPM),泛素羧基末端水解酶(UCH),牛血清白蛋白晶体结构A链(Chain A),丝氨酸蛋白酶抑制蛋白A3-5(serpin A3-5)。其中,AGP、GFAP、serpin A3-5、NPM和UCH在囊肿组织中高表达,Chain A只在正常组织中表达。通过分析这些蛋白在组织间的表达差异,发现其可能与黄体囊肿的发生和囊肿发生后激素分泌异常有关。黄体囊肿差异蛋白的发现为奶牛黄体囊肿临床诊断及发生机理的研究提供了科学依据。

In order to study the pathogenesis and biomarkers of luteal cysts in dairy cows,proteomics classic twodimensional gel electrophoresis（ 2- DE） was used to detect and analyze the differences in proteins of luteal cysts and normal luteal tissues. PDQuest8. 0 software was employed to detect and analyze protein spots after brilliant blue staining.Six proteins were identified by MALDI TOF / TOF-MS / MS tandem mass spectrometry,namely： α- 1- acid glycoprotein（ alpha- 1- acid glycoprotein,AGP）,glial fibrillary acidic protein（ glial fibrillary acidic protein,GFAP）,nuclear phosphoprotein（ nucleophosmin,NPM） ubiquitin carboxy-terminal hydrolase（ ubiquitin carboxyl-terminal hydrolase,UCH）,bovine serum albumin crystal structure A chain（ Chain A,Crystal Structure Of Bovine Serum Albumin In）,a serine protease inhibitor protein A3- 5（ serpin A3- 5）. Alpha- 1- acid glycoprotein,glial fibrillary acidic protein,nucleophosmin,ubiquitin carboxyl-terminal hydrolase,serpin A3- 5 were highly expressed in the tissue cysts,whereas,bovine serum albumin crystal structure A chain was found expressed only in normal tissue. Through analyzing the expression of these six proteins,it was found these proteins may be related to corpus luteum cyst and then hormone abnormalities. The findings of differentially expressed proteins in luteal cysts,not only provides theoretical basis for clinical diagnosis of corpus luteum cysts in dairy cows,but also for discovering and mechanisms of luteal cysts.