HMW-GS对春小麦品质的影响及不同亚基评分比较

Effects of HMW-GS on Quality Properties of Spring Wheat and Evaluation of Subunit Score System

为了明确高分子麦谷蛋白亚基(HMW-GS)对春小麦品质的影响,以及不同亚基评分方法的准确性,对22份春小麦的亚基组成和数目与小麦品质的关系进行研究,并对不同亚基的评分方法进行对比。结果表明,Glu-A1位点Null的小麦品质明显低于2*亚基和1亚基。Glu-B1位点17+18和7+8及7+9亚基的差异对小麦品质影响不大,仅在面筋指数上17+18亚基优于7+8亚基,在延伸度(L)上17+18亚基显著优于7+9亚基。Glu-D1位点等位基因的变异对小麦品质的影响较大,5+10亚基明显优于2+12亚基,在面筋指数、颗粒度、灰分、P、W、IE、PRMAX、WA上差异显著或极显著。综合评价,Glu-1位点对小麦品质的贡献大小顺序依次为Glu-D1>Glu-A1>Glu-B1。在4种亚基评分方法中Payne评分方法与春小麦的品质相关性最好。本研究结果为春小麦品质的育种早代选择提供了一定的理论依据。

To understand the effects of HMW-GS on quality properties in spring wheat and correlation of different score system of HMW-GS,the relationship between HMW-GS composition or number and quality properties in spring wheat was studied,the different score system of HMW-GS were evaluated. The results show that the wheat quality properties in spring wheat which have Null subunit are worse than those have 2＊ subunit or 1 subunit. Glu-B1 loci have little effect on quality properties of spring wheat. 17 ＋ 18 subunit was just better than 7 ＋ 8 subunit in the gluten index and than 7 ＋9 subunit in extensibility（ L）. There was obvious effect of the different HMW-GS composition on wheat properties in GluD1 loci. 5 ＋ 10 subunits were better than 2 ＋ 12 subunits; there were difference or significant difference in gluten index,granularity,ash,P,W,IE,PRMAX and WA. Based on analysis,the contribution in Glu-1 loci to the sping wheat quality properties was Glu-D1 〉 Glu-A1 〉 Glu-B1 in Glu-1. The Payne’s score system was the most correlative in four score systems of HMW-GS. The results could provide useful reference for early generation selection in spring wheat breeding.