摘要
为了提高葡萄加工副产物的综合利用率,以巨峰葡萄为原料,分离葡萄皮和籽中的游离酚与结合酚,采用HPLC法分析其组成及含量,并通过DPPH和FRAP法比较其体外抗氧化活性。结果表明,葡萄皮游离酚以儿茶素为主,籽游离酚以儿茶素和表儿茶素为主;葡萄皮和籽中结合酚均以绿原酸和表儿茶素为主;4种多酚均表现出明显的抗氧化活性,其活性大小为籽游离酚>皮游离酚>皮结合酚>籽结合酚。本研究结果为葡萄加工副产物资源的合理利用提供了数据支持。
In order to improve the utilization of by-products from grape,free and bound phenolics were extracted from kyoho grape peel and seed. The individual compounds of free and bound phenolics were analyzed by HPLC,and the antioxidant capacity of phenolics was determined by DPPH and FRAP methods. The results showed that catechin was the major compound in free phenolics from grape peel. Epicatechin and catechin were the major compounds in free phenolics from seeds. Chlorogenic acid and epicatechin were the major compounds in bound phenolics. The significant antioxidant capacity was detected in free and bound phenolics. The level of antioxidant activity of grape polyphenols was as following： free phenol in seed 〉 free phenol in peel 〉 bound phenolic in peel 〉 bound phenol in seed. This study will provide a basic data support for the application of grape by-products.
出处
《核农学报》
CAS
CSCD
北大核心
2017年第1期104-109,共6页
Journal of Nuclear Agricultural Sciences
基金
山东省农业重大应用技术创新课题(葡萄全果资源化利用与绿色防腐保鲜关键技术研究)
公益性行业(农业)科研专项经费(201303076-06)
关键词
葡萄
游离酚
结合酚
抗氧化活性
grape
free phenolics
bound phenolics
antioxidant activity
