七十味珍珠丸抗大鼠脑缺血再灌注损伤的量效关系研究

Dose-effect relationship of Qishiwei Zhenzhu Pills against cerebral ischemicreperfusion injury in rats

目的揭示七十味珍珠丸抗大鼠脑缺血再灌注损伤的量效关系。方法制备大鼠脑缺血再灌注损伤模型,七十味珍珠丸用生理盐水配制成3 mg/m L混悬液,造模后立即ig给药,于脑缺血2、24 h分别检测大鼠脑梗死率、神经行为学、体质量和血液生化指标,并分析给药剂量与脑梗死率之间的量效关系。结果七十味珍珠丸4.17、8.33、16.67、33.34、66.68、133.36 mg/kg组大鼠脑梗死率明显降低(P<0.01、0.05),且与药物剂量呈负相关(P<0.05,相关系数为-0.689),达峰剂量(Dmax)为33.34 mg/kg,最大效应(Emax)即脑梗死率最小为3.02%。七十味珍珠丸对大鼠神经行为学异常、体质量无影响。七十味珍珠丸0.52、1.04、2.08、4.17、33.34 mg/kg组血清丙二醛(MDA)水平明显降低(P<0.05)。七十味珍珠丸33.34、66.68、133.36 mg/kg组血清中超氧化物歧化酶(SOD)活力明显升高(P<0.01、0.05)。结论七十味珍珠丸能降低大鼠脑梗死率,有效剂量4.17~133.36 mg/kg,最大有效剂量33.34 mg/kg,可能与降低MDA水平,升高SOD活力有关。

Objective To reveal the dose-effect relationship of Qishiwei Zhenzhu Pills in treatment of cerebral ischemic- reperfusion injury in rats. Methods Cerebral ischemic-reperfusion injury model in rat was established. Qishiwei Zhenzhu Pills （3 mg/mL suspended in normal saline） were ig administered after model established. Then cerebral infarction rate, nerve behavior, body weight, and blood biochemical indexes were tested at 2 and 24 h, respectively. And an analysis of dose-effect relationship between dose and rate of cerebral infarction was carried out. Results Cerebral infarction rates in Qishiwei Zhenzhu Pills group （4.17, 8.33, 16.67, 33.34, 66.68, and 133.36 mg/kg） were obviously reduced （P 〈 0.01, 0.05）, and there was a clear negative correlation between dose and rate of cerebral infarction （P 〈 0.05）, and the correlation coefficient was -0.689. Qishiwei Zhenzhu Pills reached its Dmax of 33.34 mg/kg with Emax ie minimum rate of cerebral infarction 3.02%. There was no significant effect for Qishiwei Zhenzhu Pills on nerve behavior and body weight in rats. MDA contents in Qishiwei Zhenzhu Pills group （0.52, 1.04, 2.08, 4.17, and 33.34 mg/kg） were decreased （P 〈 0.05）, but total SOD activities in Qishiwei Zhenzhu Pills group （33.34, 66.68, and 133.36 mg/kg） were increased （P 〈 0.01, 0.05）. Conclusion Qishiwei Zhenzhu Pills can reduce the rate of cerebral infarction, and the effective dose range was from 4.17 to 133.36 mg/kg with its maximum effective dose of 33.34 mg/kg, which may be related to MDA content and SOD activity.