桂皮醛抑制血管内皮细胞与中性粒细胞黏附及其分子机制

Inhibition effect and molecular mechanisms of cinnamaldehyde on the adhesion of polymorphonuclear cells to HUVECs

目的:研究桂皮醛对白细胞与血管内皮细胞黏附影响的分子机制,探讨其促进皮肤创伤愈合的机制。方法:虎红染色法观察桂皮醛高(10mg/L)、中(5 mg/L)、低(2.5 mg/L)浓度对人中性粒细胞(polymorphonuclear,PMN)与肿瘤坏死因子(tumor necrosis factor,TNF)诱导的人脐静脉内皮细胞(human umbilical vein endothelial cell,HUVEC)黏附的影响;荧光免疫细胞化学法观察桂皮醛对HUVEC表面细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)、血管细胞黏附分子-1(vascular cell adhesion molecule-1,VCAM-1)和CD_(44)表达的影响。结果:桂皮醛和TNF共同作用HUVEC 4 h后,高浓度桂皮醛能明显抑制HUVEC与PMN黏附(P<0.05),能明显抑制HUVEC表面VCAM-1表达(P<0.01)。中浓度桂皮醛能明显抑制HUVEC表面ICAM-1和VCAM-1表达(P<0.05)。各浓度桂皮醛对HUVEC表面CD_(44)表达未表现明显作用。桂皮醛和TNF共同作用HUVEC 12 h后,桂皮醛未表现以上作用。结论:桂皮醛作用早期通过降低TNF诱导的HUVEC表面黏附分子的表达,抑制白细胞与血管内皮细胞的黏附,从而抑制过度的炎症反应来促进创伤愈合。

AIM： To observe the inhibition effects and molecular mechanisms of cinnamaldehyde on the adhesion of polymorphonuclear（PMN） to hu- man umbilical vein endothelial cells （HUVEC）, and to explore the mechanism of cinnamaldehyde on promoting skin wound healing. METHODS： The effect of cinnamaldehyde on HUVEC and human pe- ripheral PMN cell activity were observed by MTT and trypan-blue assay, repectively. The effect of cinnamaldehyde at high （ 10 mg/L ）, medium （ 5 mg/L）and low（2.5mg/L）concentration on the adhesion of PMN to HUVEC primed by TNF were observed by Rose Bengal Stainning. Expressions of ICAM-1, VCAM-1and CD44 on HUVEC were meas- ured by fluorescent-immunocytochemistry assay. RESULTS： High dose of cinnamaldehyde decreased PMN-HUVEC adhesion at 4 h after treated by cinna- maldehyde and TNF（P 〈 0.05 ）, and showed lower level of VCAM-1 on HUVEC （P 〈 0.01 ）. Medium dose of cinnamaldehyde decreased the level of ICAM-1 and VCAM-1 on HUVEC （P 〈 0.05）. There was no statistic difference for cinnamaldehyde on the level of CD44 on HUVEC. CONCLUSION： Cinnamaldehyde may inhibit the adhesion of PMN to HUVEC by decreasing the expression of adhesion molecules on HUVEC, therefore inhibiting excessive inflammatory response.