林下山参冻干粉的急性毒性和遗传毒性研究

Study on acute toxicity and genetic toxicity of freeze-dried powdered mountain cultivated ginseng

目的研究林下山参冻干粉的急性毒性和遗传毒性。方法采用最大耐受剂量法进行小鼠急性毒性试验,通过Ames试验、小鼠骨髓嗜多染红细胞微核试验和小鼠精子畸形试验检测林下山参冻干粉的遗传毒性。结果雌、雄小鼠对林下山参的最大耐受量(MTD)均>15.00 g/(kg·BW),属于无毒级物质;各剂量组的TA97、TA98、TA100、TA102菌株的回变菌落数与溶剂对照组比较,回变菌落数均未超过自发回变菌落数的1倍,亦无剂量-反应关系,结果为阴性;小鼠骨髓嗜多染红细胞微核试验和小鼠精子畸形试验中各剂量组微核率、精子畸形率与阴性对照组之间差异无统计学意义(P>0.05),而环磷酰胺阳性对照组与阴性对照组之间差异有统计学意义(P<0.01),显示试验结果均为阴性。结论在该试验条件下,林下山参冻干粉属于无毒级物质,亦不具有遗传毒性。

Objective To study the acute toxicity and genetic toxicity of freeze- dried powdered mountain cultivated ginseng.Methods Acute toxicity test for mice was done by test of maximum tolerated dose（ MTD）. Genetic toxicity was detected by ames test,micronucleus test of bone marrow PCE cell in mice and mice sperm abnormality test. Results The MTD of freeze-dried powdered mountain cultivated ginseng for mice were more than 15. 00 g /（ kg·BW）,it was a substance without toxicity.Comparing with the solvent control group,the reverse mutations of S. typhimurium TA97,TA98,TA100,TA102 were not one time more than nature mutations,and no dose- response relationship was found. Negative results appeared in ames test. Comparing with the negative control group,there were no statistical significance on the differences in micronucleus rate of micronucleus test of bone marrow PCE cell in mice and the teratospermia rate of mice sperm abnormality test（ P〉0. 05）,but there was statistical significance on the differences between the positive control groups of cyclophosphamide and negative control groups（ P〈0. 01）,demonstrating that test results were negative. Conclusion Under this experimental condition,freeze- dried powdered mountain is a non- toxic substance,and it has no genetic toxicity.