三焦针法对阿尔茨海默病免疫功能影响的研究

Clinical study on the effect of Sanjiao acupuncture on immune function in Alzheimer's disease

[目的]观察三焦针法对阿尔茨海默病(AD)的临床疗效及对患者免疫功能的影响。[方法]收集AD患者32例和健康老年人30例。AD组给予三焦针法,取穴膻中、中脘、气海、血海、足三里和外关,健康组不给予任何干预。利用简易精神状态检查表(MMSE)、临床痴呆量表(CDR)、老年性痴呆评估量表-认知分量表(ADAS-cog)和日常生活活动量表(ADL)评价AD患者针刺前后的认知功能、非认知功能和日常生活能力。应用流式细胞术和酶联免疫吸附法(ELISA法)检测外周血CD3^+、CD3^+CD4^+、CD3^+CD8^+T细胞数量、CD4^+/CD8^+比例、免疫球蛋白(IgG)、白介素(IL)-1β、IL-2、IL-6、干扰素-γ(IFN-γ)的水平。[结果]针刺后AD组MMSE评分升高,CDR、ADAS-cog、ADL评分降低(P<0.05)。与健康组比较,AD组CD3^+、CD8^+T细胞数量减少,CD4^+T细胞数量增加(P<0.05),针刺后CD3^+T细胞数量增加(P<0.05)。与健康组比较,AD组IL-1β、IL-2、IL-6、IFN-γ水平增加(P<0.05),IgG未见明显改变(P>0.05),针刺后以上细胞因子水平降低(P<0.05)。[结论]三焦针法可能通过调节AD患者的免疫功能而改善其痴呆状态。

Objective] To observe the clinical therapeutic effect on Alzheimer’s disease （AD） and the effect of immune function of AD patients by Sanjiao acupuncture. [Methods] The 32 cases of healthy old people and 30 cases of AD patients were collected. AD patients were acupunctured by Sanjiao acupuncture, with the acupoints of Shanzhong, Zhongwan, Qihai, Xuehai, Zusanli and Waiguan. Healthy old people group was just as control. Mini-mental state examination （MMSE）, clinical dementia rating scale （CDR）, the geriatric dementia assessment scale （ADAS-cog） and the daily living activity scale （ADL） were used to evaluate the cognitive function, the non-cognitive function and the daily living ability of patients respectively. And flow cytometry and ELISA assay were used to detect CD3＋, CD3＋CD4＋, CD3＋CD8＋T cell numbers, CD4＋/CD8＋ratio, and IgG, IL-1β, IL-2, IL-6, IFN-γlevel. [Results] After acupuncture, MMSE score of AD group was increased, and CDR, ADAS-cog and ADL score was decreased （P〈0.05）. In AD group, the numbers of CD3＋and CD8＋T cells were decreased compared to the healthy group, while the number of CD4＋T cells was increased. After acupuncture, the number of CD3＋T cells was increased （P〈0.05）. When compared to the healthy group, the levels of IL-1β, IL-2, IL-6, IFN-γin AD group were increased （P〈0.05）, while IgG was not significantly changed （P﹥0.05）, and after acupuncture the levels of cytokines were decreased （P〈0.05）. [Conclusion] Sanjiao acupuncture could improve dementia symptoms of AD patients, which might by related to regulating the immune function of patients.