甲巯咪唑致亚临床甲减大鼠模型评价

Evaluation of Methimazole induced subclinical hypothyroidism rat model

目的探讨应用甲巯咪唑(MMI)建立亚临床甲减(SCH)大鼠模型的有效给药剂量及时间点。方法将45只成年雄性Wistar大鼠随机分为3组:正常对照组;MMI低剂量组予MMI 5 mg/(kg·d)灌胃;MMI高剂量组予MMI 10 mg/(kg·d)灌胃,分别于给药后第45、90、120天采血测定甲状腺功能。结果 45 d时MMI高剂量组血清TSH水平与正常对照组比较差异有统计学意义(P<0.05),符合SCH标准的有7只,成功率为63%(7/11)。90 d时MMI低剂量组和MMI高剂量组分别与正常对照组比较:血清TSH水平差异均有统计学意义(P<0.05),90 d时MMI高剂量组符合SCH诊断标准有8只,成功率为66%(8/12),MMI低剂量组符合SCH的诊断标准有6只,成功率为60%(6/10)。120 d时MMI低剂量组和MMI高剂量组分别与正常对照组比较:血清TSH及T4水平差异均有统计学意义(P<0.01)。120 d时MMI高剂量组符合SCH诊断标准有1只,成功率为8%(1/12),MMI低剂量组符合SCH的诊断标准有4只,成功率为33%(4/12)。结论应用MMI可以成功建立SCH大鼠模型。

Objective To investigate the effective dose and time point of methimazole（MMI） on subclinical hypothyroidism（SCH） rat model. Methods Forty five adult male Wistar rats were randomly divided into three groups：normal control group; MMI low dose group received MMI 5mg/（kg·d）gavage; MMI high dose group received MMI 10mg/（kg·d）gavage, respectively to the drug after the 45 days, 90 days and 120 days blood determination of thyroid function. Results 45 days MMI high-dose group, the serum TSH levels compared with normal control group, the difference has statistical significance（P 0.05）, accord with the standard of SCH 7 only, the success rate was 63%（7/11）. 90 days MMI low dose group and MMI high dose group compared with the normal control group：serum TSH levels were statistically significant difference（P 0.05）.At 90 days MMI high dose group in accordance with the SCH diagnostic criteria has 8, the success rate of 66%（8/12）, MMI low dose group in accordance with the diagnostic criteria of SCH has 6, the success rate of 60%（6/10）. 120 days MMI low and high dose group compared with the normal control group：serum TSH levels and serum T4 were statistically significant difference（P〈0.05）. At 120 days MMI high dose group in accordance with the SCH diagnostic criteria has 1, the success rate of 8%（1/12）. MMI low dose group in accordance with the diagnostic criteria of SCH has, the success rate of 33%（4/12）. Conclusion SCH rat model can be established by using MMI.