HPLC法测定蜂胶类保健食品中黄曲霉毒素B_1的研究

Study of Determination for Aflatoxin B_1 in Propolis Related Health Food by HPLC

建立蜂胶类保健食品中黄曲霉毒素B_1的含量测定方法,完善相关保健食品中黄曲霉毒素B_1的质量控制。色谱柱:Athena C_(18)-WP色谱柱(4.6 mm×250 mm×5μm);流动相为水-甲醇-乙腈(体积比57∶26∶17);流速:0.8m L/min;柱温:30℃;荧光检测器激发波长:360 nm;发射波长450 nm。结果表明,黄曲霉毒素B_1在0.32~24.62 ng/m L范围内线性关系良好(R^2=0.999 7),其加样回收率为79.5%~95.0%,方法的最低检出限为1.5μg·kg^(-1)。该法简便、可靠、准确,可用于蜂胶类保健食品中黄曲霉毒素B1的含量测定。

To establish HPLC method for the determination ofaflatoxin B1 in propolis related health food, improve the quality control of aflatoxin B1 in propolis related health food. Agilent Cls （4.6 mm×250 mm, 5 μm） column was used with column temperature at 30 ℃. The mobile phase consisted of water, methanol and acetonitrile, the proportion was 57 ： 26 ： 17, respectively, and the flow rate was 0.8 mL/min. The fluorescence detector was used in the study, condition was below： excitation wavelength 360 nm, emission wavelength 450 nm. The results showed that linear relationship of aflatoxin B1 showed good at 0.32-24.62 ng/mL （R^2=0.999 7）, its recovery was 79.5%-95.0%, the detection limit of aflatoxin Bl was 1.5 μg·kg^-1. The method was simple, reliable and accurate, and it could be used for the content determination of aflatoxin B1 in propolis related health food.