细菌重组系统及其应用研究进展

Progress on bacterial recombination systems and their application

利用重组酶和辅助蛋白共同作用于DNA片段上,使不同基因重新组合以完成基因重组的现象在细菌中广泛存在,基因重组对于细菌的遗传多样性、进化等具有重要意义。目前,细菌基因重组主要分为同源重组、位点特异性重组和转座重组3种类型。本文主要对细菌重组系统重组酶的种类、作用机制及其在细菌遗传操作中的应用策略进行阐述。

Gene recombination through recombinases and auxiliary protein enzymes exists widely in bacteria and it has great significance to bacterial genetic diversity and evolution. At present, genetic recombinations in bacteria are classed into three types： homologous recombination, site-specific recombination and transposition recombination. In this article, recombinases, and related protein enzymes of bacterial recombination systems, the mechanisms of various recombinations, and applications of recombination systems to genetic manipulation were reviewed.