摘要
2015年,福建省福州地区2个鸭场(M18肉鸭场及蛋鸭场)发生大量鸭死亡,初诊疑似鸭瘟。为明确其病原,分别采集病死鸭肝脏、食道样品进行鸭瘟病毒特异性PCR检测和病毒分离,对获得的2株病毒分离株分别进行鸭已知病原的检测,确定均为鸭瘟病毒。对新分离鉴定的2株鸭瘟病毒株的UL2基因进行序列测定及分析发现,均具有强毒株的分子特征;经TK基因序列测定及分析表明,2株新分离的鸭瘟病毒株与强毒代表株、弱毒疫苗株的TK基因核苷酸同源性均高达98.7%。
In March 2015,massive deaths happened to the M18 Duck Farm and an egg-laying duck farm in Fuzhou.It was suspected of an outbreak of duck plaque.Samples of liver and esophagus from the dead birds were collected for pathogenic identification using PCR and virus isolation.Two strains with virulent characteristics were thus identified.Subsequently,a sequence analysis on their UL2 genes further confirmed the classification.In addition,the TK gene sequences of the two isolated virus strains showed a nucleotide homology of 98.7% with those of the common virulent strain as well as the vaccine strain.
出处
《福建农业学报》
CAS
北大核心
2016年第12期1257-1261,共5页
Fujian Journal of Agricultural Sciences
基金
国家水禽产业技术体系项目(CARS-43)
福建省畜禽疫病防控技术重大研发平台(2014N2003)
福建省科技计划项目--省属公益类科研院所基本科研专项(2017R1102)
福建省农业科学院科研项目(2015QC-2)
"国家百千万工程人才"科研补助项目(NCNCMTPC-2009)
关键词
鸭瘟病毒
分离鉴定
UL2基因
TK基因
序列分析
virulent duck plague virus
isolation and identification
UL2 gene
TK gene
sequence analysis
