利用物质组学方法筛选产游离油酸的芽胞杆菌菌株

Oleic-acid-producing Bacillus-like Bacteria Screened by Means of Chemomics

利用高效液相色谱-四级杆飞行时间质谱联用技术测定了52个芽胞杆菌Bacillus-like菌株发酵上清液中的代谢物组成,各物质的分子特征通过MassHunter软件从原始数据中进行提取,然后通过Metlin代谢物质谱数据库的检索比对,获得各代谢物的基本信息。结果表明,在海绵芽胞杆菌Bacillus plakortidis菌株FJAT-14858的发酵上清液中,可以检测出游离油酸,其Metlin匹配得分达95.61,其相对含量占发酵上清液总代谢物的1.62%,精确质量数为284.269 3。海绵芽胞杆菌菌株FJAT-14858是首个被发现产非毒性的游离油酸的芽胞杆菌菌株,本研究为新型微生物油脂的开发与利用提供菌种资源和理论依据。

Extracellular metabolites in the supernatants 52 Bacillus-like strains were analyzed by HPLC along with the quadrupole time-of-flight mass spectrometry（HPLC-Q-TOF-MS）.Subsequently,the molecular feature extraction（MFE）was performed on the data using the software MassHunter followed by submitting the information for comparison against the mass spectrometry database,Metlin.Free oleic acid was thereby detected in the cultural supernatant of Bacillus plakortidis,FJAT-14858.Its matching score with oleic acid was 95.61,accurate mass was284.2693,and relative content in the metabolites collected was up to 1.62%.To date,B.plakortidis FJAT-14858 was the first Bacillus-like bacterium known to produce free oleic acid with no cytotoxicity to the bacteria per se.