RP-HPLC法同时测定桑杏口服液中芦丁、橙皮苷、牛蒡苷和甘草苷的含量

Simultaneous Determination of Rutin,Hesperidin,Arctiin and Liquiritin in Sangxing Oral Liquid by RP-HPLC

目的:建立同时测定桑杏口服液中芦丁、橙皮苷、牛蒡苷、甘草苷含量的方法。方法:采用反相高效液相色谱法。色谱柱为Kromasil C18,流动相为乙腈-0.1%磷酸溶液(梯度洗脱),流速为1.0 m L/min,检测波长为276、348 nm,柱温为30℃,进样量为5μL。结果:芦丁、橙皮苷、牛蒡苷、甘草苷检测质量浓度线性范围分别为1.068~10.68μg/m L(r=0.999 3)、9.320~93.20μg/m L(r=0.999 2)、46.91~469.1μg/m L(r=0.999 4)、0.511 0~5.110μg/m L(r=0.999 2);精密度、稳定性、重复性试验的RSD<3%;加样回收率分别为95.10%~101.90%(RSD=2.1%,n=9)、96.75%~101.80%(RSD=1.4%,n=9)、95.69%~100.00%(RSD=1.5%,n=9)、98.22%~104.60%(RSD=2.1%,n=9)。结论:该方法准确、灵敏、重复性好,可用于桑杏口服液中芦丁、橙皮苷、牛蒡苷、甘草苷含量的同时测定。

OBJECTIVE ：To establish a method for the simultaneous determination of rutin,hesperidin,arctiin,liquiritin and glycyrrhizinate in Sangxing oral liquid. METHODS ：RP-HPLC was performed on the column of Kromasil C18 with mobile phase of acetonitrile-0.1 % phosphoric acid solution（gradient elution） at a flow rate of 1.0 m L/min,the detection wavelength was 276,348 nm,column temperature was 30 ℃,and the injection volume was 5 μL. RESULTS：The linear range was 1.068-10.68 μg/m L（r=0.999 3）for rutin,9.320-93.20 μg/m L（r=0.999 2）for hesperidin,46.91-469.1 μg/m L（r=0.999 4）for arctiin,0.511 0-5.110μ g/m L（r=0.999 2） for liquiritin;RSDs of precision,stability and reproducibility tests were less than 3%;recoveries were95.10%-101.90%（RSD=2.1%,n=9）,96.75%-101.80%（RSD=1.4%,n=9）,95.69%-100.00%（RSD=1.5%,n=9）,98.22%-104.60%（RSD=2.1%,n=9）,respectively. CONCLUSIONS：The method is accurate,sensitive and reproducible,and can be used for the simultaneous determination of rutin,hesperidin,arctiin and liquiritin in Sangxing oral liquid.