环丙沙星对小鼠肝细胞自噬研究

The influence of ciprofloxacin on the expression of autophagy and autophagy-related genes in mouse hepatocytes

目的研究环丙沙星对小鼠肝细胞的自噬作用及基因表达。方法小鼠随机分为对照组和实验组,实验组小鼠给药环丙沙星1周,采用自噬溶酶体荧光探针检测小鼠肝细胞自噬溶酶体,利用全基因组表达谱基因芯片技术筛选差异基因。结果自噬溶酶体荧光探针检测到实验组小鼠肝细胞荧光强度降低,全基因组表达谱芯片技术发现Akr1b7/Pfkfb3调节果糖及甘露糖代谢信号通路有统计学显著差异(P<0.05)。结论环丙沙星(13.5g/kg)抑制小鼠肝细胞自噬,Pfkfb3基因可能是环丙沙星给药后影响自噬以及糖代谢的靶点之一,并且可能是通过AMPK/m TORC1途径影响自噬。

Objective To investigate the effects of ciprofloxacin on mouse hepatocytes about autophagy and autophagy-related gene by Gene Microarray Chip. Method Mice were randomly divided into experimental group that was given ciprofloxacin for one week and control group that was given saline for one week in parallel. The autophagy-related lysosome was detected by fluorescence probe. Differential gene expression was detected with mRNA rnicroarray. Results The decreased fluorescent strength of the autophagy in hepatocytes in the experimental group was detected by fluorescence probe. The mRNA expression of AkrlbT/Pfkfb3 had significant difference in the regulation of the fructose metabolism. Conclusion Ciprofloxacin （13.5g/kg） induced the inhibition of the autophagy in mouse hepatocytes. The Pfkfb3 was one of the possibe target route of the autophagy.