2-甲氧基雌二醇联合冬凌草甲素对胃癌SGC-7901细胞增殖和凋亡的影响

Effect of 2-methoxy estradiol and oridonin on proliferation and apoptosis of gastric cancer SGC-7901 cells in vitro

目的:研究2-甲氧基雌二醇(2-ME)单独和联合冬凌草甲素对胃癌SGC-7901细胞增殖和凋亡的影响。方法:用0.125、0.250、0.500、1.000、2.000、4.000、8.000、16.000μmol/L 2-ME处理SGC-7901细胞24、48和72 h后,观察细胞形态变化,SRB法检测2-ME对SGC-7901细胞增殖的抑制作用;用0.625、2.500、10.000、20.000μmol/L 2-ME以及相同浓度的冬凌草甲素单独或联合应用处理SGC-7901细胞48 h后,计算结合指数,用流式细胞术和荧光染色法观察细胞凋亡情况。结果:2-ME作用于SGC-7901细胞48 h的IC50值为5.31μmol/L,对细胞SGC-7901的抑制作用呈时间依赖性,与冬凌草甲素联合应用的IC50值为8.46μmol/L。不同浓度2-ME作用48 h,随着剂量增加,SGC-7901细胞凋亡率增加,联合应用冬凌草甲素对细胞凋亡的影响未表现出协同作用。结论:2-ME可以抑制SGC-7901细胞的增殖,诱导其凋亡,与冬凌草甲素联合应用时对肿瘤细胞增殖的抑制表现为相加作用,对凋亡未表现出协同作用。

Aim： To study the effect of 2-methoxy estradiol（2-ME）separate and combined with oridonin treatment on proliferation and apoptosis of gastric cancer strain SGC-7901 in vitro.Methods： SGC-7901 cells were treated with 0.125,0.250,0.500,1.000,2.000,4.000,8.000,16.000 μmol/L 2-ME for 24, 48 and 72 h, cells morphological changes were observed,and SRB assay was applied to detect the SGC-7901 cell growth; with different concentration（0.625,2.500,10.000,20.000 μmol/L）2-ME and the same concentration oridonin separately treatment on SGC-7901 cells for 48 h, the combination index was calculated,and the apoptosis of the cells by flow cytometry and fluorescent staining was observed.Results： The IC50 of 2-ME acted on SGC-7901 cells was 5.31 μmol/L, and the inhibitory effect of 2-ME on SGC-7901 cells was concentration- and time- dependent. After being treated by different concentrations of 2-ME for 48 h, the apoptosis rate of SGC-7901 cells was increased with the increase of the dose, and the effect of 2-ME on apoptosis had no synergistic.Conclusion： 2-ME can inhibit the proliferation of SGC-7901 cells, induce apoptosis, and enhance the effect of oridonin.