培育蝉花改善小鼠睡眠功能的试验研究

Experimental study of effect for synnema and mycelial of Isaria cicadae on sleeping

观察不同剂量的人工培养蝉花子实体及菌丝体对小鼠睡眠的改善作用,健康昆明小鼠,随机分成8组,每组10只.每天经口灌胃给予不同剂量的人工培养蝉花子实体及蝉花菌丝体,连续给予受试物30 d后进行直接睡眠作用试验、延长戊巴比妥钠睡眠时间实验、戊巴比妥钠阈下剂量催眠实验和巴比妥钠睡眠潜伏实验测定小鼠直接睡眠时间、戊巴比妥钠诱导的睡眠时间及阈下剂量的睡眠发生率、巴比妥钠诱导的睡眠潜伏时间.结果表明,与空白对照组比较人工培养蝉花子实体300 mg/kg、900mg/kg及菌丝体900 mg/kg对阈下剂量戊巴比妥钠诱导的小鼠睡眠一定的促进作用(P<0.05),且没有明显的剂量依赖关系;与空白对照比较,300 mg/kg人工培养蝉花子实体和900 mg/kg蝉花菌丝体对戊巴比妥钠致小鼠睡眠时间有明显的延长作用,但不同剂量的两种受试药物对巴比妥钠睡眠潜伏期基本无影响,且均无直接至眠作用.人工培养蝉花子实体和蝉花菌丝体对小鼠睡眠有一定的改善作用.

To observe the effects of synnema and mycelial of Isaria cicadae improve the role of sleep in mice, Kunming mice were randomly divided into 8 groups, 10 for every group. Per day orally given different doses of synnema and mycelial of Isaria cicadae, after continuous administration of the test substance of 30 days, the experiments were performed, incliding direct effects of sleep test, extend pentobarbital sleep time experiment, direct s!eep threshold dose of pentobarbital sodium pentobarbital sodium hypnotic sleep latency test and mice experimentally determined time, pentobarbital sodium- induced sleep time subthreshold dose of sleep and the incidence of sodium pentobarbital - induced sleep latency. Results indicated that compared with control group, the group synnema of Isaria cicadae （300 mg/ kg; 900 mg/kg） and mycelial of lsaria cicadae （900 mg/kg） certain role for subthreshold dose of pentobarbital sodium - induced sleep in mice （ P 〈 0.05 ） and no clear dose - de- pendent manner. The control compared with control group, the group of synnema of Isaria cicadae （300 mg/kg; 900 mg/kg） and mycelial of Isaria cicadae （900 mg/kg） pentobarbital induced sleep time in mice significantly prolonged action. However, two different doses of the test drug sodium pentobarbital no effect on sleep latency, and there were no direct role to sleep. The synnema and mycelial of Isaria cicadae have some improvement on mice sleep.