表面等离子共振技术快速检测食品中的玉米赤霉烯酮毒素

Rapid detection of zearalenone in food by surface plasmon resonance technology

目的建立基于表面等离子共振(surface plasmon resonance,SPR)技术快速检测食品中玉米赤霉烯酮毒素(zearalenone,ZEN)的方法。方法采用表面自组装技术(self-assembled monolayer,SAM)在金膜的表面修饰羧基基团,将ZEN抗原与牛血清白蛋白(albumin from bovine serum,BSA)偶联物(ZEN-BSA)通过共价键固定在芯片的表面,采用竞争法检测样品中的玉米赤霉烯酮毒素。结果该方法的检测限为8.2 ng/m L,ZEN单克隆抗体与呕吐毒素、黄曲霉毒素B_1、赭曲霉毒素、伏马毒素等没有交叉反应,与α-玉米赤霉烯醇和β-玉米赤霉烯醇交叉反应率分别为15.3%和11.5%。结论本方法具有简便、快速和高灵敏度等优势,在食品中真菌毒素的快速检测方面具有潜在的应用价值。

Objective To establish a method for the rapid detection of zearalenone （ZEN） by surface plasmon resonance （SPR） technology.Methods The surface of the gold film was modified with carboxy group by self-assembled monolayer （SAM）. The ZEN-BSA conjugate was immobilized on SPR chip through covalent bond, and the competition method was used to detect ZEN in food.Results The detection limit of this method was 8.2 ng/mL. The ZEN monoclonal antibody did not show cross reaction with deoxynivalenol, aflatoxin B1, ochratoxin and fumonisin, and the cross reaction rates withα-zearalenol andβ-zearalenol were respectively 15.3% and 11.5%. Conclusion The established method is simple, rapid, highly sensitive, which has the potential application value in the rapid detection of mycotoxins in food.