摘要
目的：测定蒙药参竹精片中总多糖的含量。方法：采用紫外分光光度法,以葡萄糖为对照品,苯酚-硫酸为显色剂,检测波长为487nm,测定样品溶液吸光度,计算总多糖的含量。结果：葡萄糖浓度在20.04～100.21μg·m L-1范围内与吸光度呈良好的线性关系,回归方程为Y=1.1819 X-0.0507（r=0.9990,n=6）,参竹精片中总多糖的平均含量为0.5314%,平均加样回收率为99.37%,RSD为0.92%。结论：采用苯酚-硫酸法测定蒙药参竹精片中总多糖含量的方法简单、准确、重现性好,适用于参竹精片中总多糖的含量测定。
Objective： To determine total polysaccharides content of Shenzhujing Tablets. Methods： UV-vis spectrophotometry was used to determine content of total polysaccharide in Shenzhujing Tablets. The experiment was carried out with the glucose as comparison,phenol-sulfuric acid as developer. 487 nm as detection wavelength. The content of total polysaccharides was calculated by determining the absorbancy of sample solution. Results： The glucose shows a good linear relationship with absorbancy at range of 20. 04 ~ 100. 21μg. m L-1. The regression equation is Y = 1. 1819 X-0. 0507（ r =0. 9990,n = 6）. The content of total polysaccharides in Shenzhujing Tablets was 0. 5314%. The average sample recovery rate was 99. 37%（ RSD = 0. 92%,n = 6）. Conclusion： Phenol-sulfuric acid method was simple accurate,reproducible and suitable for content determination of total polysaccharides in Shenzhujing Tablets.
作者
张爱武
罗素琴
刘乐乐
周秀芳
ZHANG Ai-wu LUO Su-qin LIU Le-le et al(The Affiliated Hospital, Inner Mongolia Medical University, Hohhot 010050 China)
出处
《内蒙古医科大学学报》
2016年第4期332-334,340,共4页
Journal of Inner Mongolia Medical University
基金
呼和浩特市科技局(2011重-1-5-1)
关键词
参竹精片
总多糖
苯酚-硫酸法
含量测定
shenzhujing tablets
total polysaccharides
phenol-sulfuric acid method
content determination
