LAS共代谢降解菌的降解动力学研究

Study on degradation kinetics of co-metabolic biodegradation of linear alkylbenzene sulfonate strains

研究2株直链烷基苯磺酸钠（LAS）共代谢降解菌的降解动力学.分别对2株直链烷基苯磺酸钠共代谢降解菌克雷伯氏菌属（Klebsiella sp.）和肠杆菌属（Enterobacter sp.）进行研究,通过正交实验考察了其最佳降解条件,并进行降解动力学研究.当温度为30℃,p H值为7.5,装液量为50 m L时,最适合两株细菌降解LAS.其中菌株L-2在生长基质葡萄糖质量浓度为500 mg/L时,对50 mg/LLAS降解率为94.2%;菌株L-15在生长基质葡萄糖质量浓度为1 000 mg/L时,对50 mg/LLAS降解率为92.2%;当LAS质量浓度在25～100 mg/L范围内,2菌株降解反应符合一级动力学特征.本研究可为全基质生活污水处理LAS废水提供一定的理论依据.

The degradation kinetics of co- metabolic biodegradation of two linear alkylbenzene sulfonate（ LAS） strains was studied. Two bacterial strains which could degrade linear alkylbenzene sulfonate by co- metabolism mechanism,named as L- 2 and L- 15 were determined to Klebsiella sp. and Enterobacter sp. The optimal degradation conditions of L-2 and L- 15 were investigated by the orthogonal experiment. The results suggested that the degradation rate of LAS（ 50 mg / L） by L- 2 was up to 94. 2% when glucose was chosen as growth substrate under the conditions as： 30 ℃,p H 7. 5,glucose concentration 500 mg / L,while the degradation rate of LAS（ 50 mg / L） by L- 15 was up to 92. 2% under the conditions as： 30 ℃,p H = 7. 5,glucose concentration 1 000 mg / L. The degradation reactions fitted first order kinetics when the concentration of LAS ranged from 25 mg / L to 100 mg / L.Certain theoretical basis of the treatment of LAS wastewater was provided by this study.