摘要
目的研究潜艇密闭环境下军人皮肤表面微生物分布特征和变化特点,为潜艇艇员针对性卫勤保障提供依据。方法利用样本采集拭子采集潜艇艇员皮肤表面标本122份,进行细菌培养,分纯后通过基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF-MS)鉴定。结果共分离细菌52种229株,真菌2种2株,主要优势条件致病菌为鲍氏不动杆菌、铜绿假单胞菌和阴沟肠杆菌,优势栖居菌为滕黄微球菌、奥斯陆莫拉菌、乌尔新不动杆菌、表皮葡萄球菌和黏质沙雷菌。任务中后期与任务前期相比,优势条件致病菌和栖居菌均明显增多。结论获得了潜艇密闭环境下军人皮肤表面微生物菌谱,平战时需进行针对性药品配备并采取战创伤救治措施。
Objective To investigate the distribution and changes of microorganisms on the skin of submariners under a chronically closed environment,and provide reference for targeted medical support. Methods One hundred and twenty-two samples were collected using swab. After culture and isolation,the microbes were identified by matrix-assisted laser desorption /ionization time of flight mass spectrometry(MALDI-TOF-MS). Results A total of 52 types of 229 bacteria and 2 types of fungi were isolated. Major opportunistic pathogens included Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter cloacae, while major dwelling bacteria included Micrococcus luteus, Oslo Mora bacteria, Acinetobacter,Staphylococcus epidermidis,and Serratia marcescens. Compared with the early period of the task,major opportunistic pathogens and dwelling bacteria were significantly increased in the middle and late period of the task. Conclusion The skin microbes of the submariners are investigated,targeted drugs need to be prepared for daily medical support and war trauma.
出处
《军事医学》
CAS
CSCD
北大核心
2017年第1期21-24,共4页
Military Medical Sciences
基金
全军"十二五"后勤科研重点资助项目(BWS13L019)
全军"十二五"后勤科研面上资助项目(CWS13J039)
全军后勤计划重大专项资助项目(AWS14C003-2)