增龄性因素对正畸牙齿移动影响的实验研究

Experimental Study on Influence of Aging Factors on Orthodontic Tooth Movement

目的:探索增龄性因素对正畸牙移动过程中牙周组织内HIF-1α及VEGF的影响。方法:建立不同年龄组的正畸牙齿移动动物模型,加力1d、3d、7d、14d及28d,用免疫组化法检测牙周组织中HIF-1α及VEGF的表达情况。结果:青少年组大鼠实验侧压力区HIF-1α表达在加力1d、3d时明显高于成年组大鼠;VEGF表达量在加力1d、3d、7d、14d时明显高于成年组大鼠。结论:增龄性因素可使牙周组织对矫治力的反应速率降低,缺氧调控速率减慢,最终使牙槽骨改建减慢,牙移动速率降低。VEGF参与了正畸牙移动中组织改建过程,但成年组大鼠正畸牙齿移动过程中VEGF表达量增加缓慢,使牙槽骨改建速度降低,造成牙齿移动速度减慢。

Objective：To explore the effects of aging factors on the expression of HIF-1αand VEGF in the periodontal tissues during orthodontic tooth movement in rats.Methods：Orthodontic tooth movement animal models with different ages were established.The expressions of HIF-1αand VEGF in the periodontal tissues were detected by immunohistochemistry method at day 1,3,7,14 and 28after orthodontic force application.Results：The expression of HIF-1αin the young group was significantly higher than that in the adult group at day 1and 3.The expression of VEGF was higher than that in the adult group at day 1,3,7and 14.Conclusion：Aging factors can decrease the reaction of the periodontal tissue,and slow down the rate of hypoxia regulation.Finally,the alveolar bone remodeling and the speed of tooth movement would decrease.VEGF plays an important role in the periodontal tissue remodeling.But the expression of VEGF increases slowly in the adult group.The speed of alveolar bone reconstruction reduced,as well as the speed of tooth movement.