氟纳米羟基磷灰石对早期根面龋再矿化效果的实验研究

Effect of Fluoride Nano Hydroxyapatite on Remineralization of Early Root Caries

目的:通过实验研究氟纳米羟基磷灰石对早期根面龋的再矿化效果,并与纳米羟基磷灰石、氟化泡沫和氟化钠溶液的再矿化效果进行比较。方法:制备人工早期根面龋模型41个,使用扫描电镜观察脱矿后的根面的表面形态及用数显显微硬度计测量脱矿后根面的显微硬度。将40个根面龋模型随机分为5组:空白对照组、氟纳米羟基磷灰石组、纳米羟基磷灰石组、氟化泡沫组和氟化钠溶液组。每组使用其相应的试剂处理,经7dpH循环及20d再矿化处理,于扫描电镜观察和显微硬度计测量。统计采用单因素方差分析并作LSD-t检验,实验前后采用单样本t检验。结果:氟纳米羟基磷灰石组扫描电镜观察根表面有大量晶粒沉积且平滑,其硬度值与脱矿后和其他组硬度值比较,有统计学意义(P<0.05)。结论:氟纳米羟基磷灰石对早期根面龋有较好的再矿化效果。

Objective：To evaluate the remineralization effect of fluoride nano hydroxyapatite on early root caries and to compare the effect of remineralization between nano hydroxapatite,the fluoride foam,and the sodium fluoride.Methods：Forty-one artificial early root caries models were prepared,and the root surfaces were observed by scanning electron microscope（SEM）.After demineralization,the micro hardness of the caries was measured,and the samples were randomly divided into 5groups：the fluorine nano hydroxyapatite group,the nano hydroxyapatite group,the fluoride foam group,the sodium fluoride solution group,and the control group.The samples in each group were demineralized by pH circulation for 7days,and were remineralized by different agents for 20 days.Finally,the samples were observed by SEM and the micro hardness was tested.Results：Large grains of crystals were deposited on the root surface in the fluoride nano hydroxyapatite group,and the surface of the group was smoother than those in the other groups.Micro hardness of the fluoride nano hydroxyapatite group was statistically different from those of other groups（P〈0.05）.Conclusion：The fluoride nano hydroxyapatite could improve the remineralization of the early root caries.