补肾解毒方对辐射所致造血系统损伤的防护作用研究

Bushen Jiedu Recipe Protected Radiation Induced Hematopoietic Injury

目的研究补肾解毒方对辐射所致造血系统损伤的防护作用。方法将C57BL/10J小鼠(TLR4+/+)及TLR4基因敲除小鼠(TLR4-/-)随机分为空白对照组、辐射模型组、补肾解毒方组。分别于辐射后第1、14、30d取材,观察小鼠一般情况,检测各组小鼠红细胞数、白细胞数、血小板数;取小鼠股骨标本,制作骨髓切片,观察骨髓标本病理形态学变化。结果与空白对照组比较,辐射后1d小鼠白细胞、红细胞、血小板均下降或呈下降趋势。辐射后14d小鼠红细胞和血小板下降显著(P<0.01,P<0.05);TLR4+/+补肾解毒方组白细胞、血小板数目高于其他2组(P<0.01,P<0.05)。辐射后30d小鼠红细胞、血小板与空白对照组比较,差异无统计学意义(P>0.05),TLR4+/+补肾解毒方组血小板与正常值最为接近。白细胞明显回升,但与空白对照组,比较差异仍有统计学意义(P<0.01),TLR4+/+补肾解毒方组白细胞恢复最快;除TLR4+/+补肾解毒方组外,光学显微镜观察辐射后其他小鼠骨髓结构均遭到不同程度破坏。结论补肾解毒方能通过TLR4信号通路减轻辐射所致的造血系统损伤,起到显著的辐射防护作用。

Objective To observe the protective effects of Bushen Jiedu Recipe （ BJR） on radiation induced hematopoietic injury.Methods C5 7 BL /1 0 J mice TLR4 gene （ TLR4＋/＋） and knockout mice （ TLR4 - / - ） were randomly divided into 3 groups,the blank control group,the radiation model group,the BJR group.Sampling was respectively performed at day 1 ,1 4 ,and 3 0 after radiation.The general condition of mice was observed.White blood cell （ WBC） ,red blood cell （ RBC） ,and platelet （ PLT） were counted in each group.Thigh bone of mice was collected for bone marrow specimen.Bone marrow slice was prepared.Pathomorphological changes were observed under electron microscope.Results Compared with the blank control group,WBC,RBC,and PLT all decreased or showed a decreasing tendency after one day radiation.RBC and PLT significantly decreased after 1 4 days of radiation （P〈 0.01 ,P〈0.05 ） .Counts of WBC and RBC were higher in the TLR4＋/＋BJR group than in the rest 2 groups （P〈0.01 ,P〈0.05 ） .No statistical difference in RBC or PLT between mice after 3 0 days of radiation and mice in the blank control group （P〉0.05 ） .PLT count in the TLR4＋/＋BJR group was most approximate to the normal value.WBC count obviously increased,but still with statistical difference as compared with the blank control group（P〈0.01 ） .WBC count recovered most rapidly in the TLR4＋/＋BJR group.Results under light microscope showed the structure of bone marrow was injured to different degrees in all mice except those in the TLR4＋/＋BJR group.Conclusion BJR could attenuate radiation induced hematopoietic injury possibly through TLR4 signal pathway,thus playing significant radioprotective roles.