电针对糖尿病胃轻瘫大鼠胃窦部INS及IR表达的影响

Effects of Electroacupuncture on the Expression of INS and IR in Gastric Antrum of Diabetic Gastroparesis Rats

目的观察电针"足三里"等穴对糖尿病胃轻瘫(diabetic gastroparesis,DGP)模型大鼠胰岛素及其受体表达的影响,探讨电针调整血糖、改善DGP胃肠动力的可能机制。方法将50只SD大鼠随机分为5组,即空白对照组、DGP模型对照组、电针穴位组、电针非穴组、胃复安对照组,每组10只。造模组按55 mg/kg单次右下腹腔注射2%链脲佐菌素(STZ)联合高脂高糖不规律饮食建立DGP模型,连续喂养8周,每周用强生稳豪倍易型血糖仪及试纸测量血糖,尿糖试纸测量尿糖。电针穴位取大鼠"足三里"、"梁门"、"三阴交",电针非穴组取大鼠"足三里"、"梁门"、"三阴交"的穴位对照点,胃复安对照组予1.7%胃复安药液(1 ml/100 g)灌胃。治疗结束后,以酚红为标志物,观察各组大鼠胃排空率及小肠移行率,以ELISA法检测胃窦部胰岛素(INS)及胰岛素受体(IR)的表达。结果与空白对照组比较,模型组血糖水平、症状积分明显升高,胃排空率、小肠移行率、胃窦部INS、IR含量明显降低(P<0.01);与模型组比较,电针穴位组血糖水平及症状积分明显降低,胃排空率及小肠移行率、胃窦组织INS及IR表达升高(P<0.05,P<0.01),与电针穴位组比较,电针非穴组、胃复安对照组症状积分升高、胃窦部INS、IR含量明显减少(P<0.05,P<0.01)。结论电针能降低DGP大鼠血糖,促进DGP大鼠胃肠动力,其作用机制可能与增加胃窦部INS及IR含量有关。

Objective To observe the effects of electroacupuncture （EA） stimulation at ＂Zusanli” etc. on level of insulin （INS） and insulin-like receptor （IR） in diabetic gastroparesis（DGP） rats, so as to investigate its mechanism underlying adjusting blood glucose level and improving gastrointestinal motility. Methods Fifty Sprague-Dawley （SD） rats were randomly divided in-to five groups black control group, DGP model group, EA acupoint group, EA non-acupoint group, metocloppramide group,10 rats in each group. The DGP models were established by intraperitoneal injection of 2〉 concentration of streptozotocin （STZ, 55 mg/kg） and by feeding the rats with high fat -sugar forage for irregularly 8 weeks. Blood glucose of all rats were determined by blood glucose meters and test strips produced by American Johnson company and its urine sugar by tes-tape for every week. The EA was performed to ＂Zusanli”,＂Liangmen”,＂Sanyingjiao” and non-acupoints. The metocloppramide group were received 1.7_ concentration of metocloppramide （1 mL/100 g）. After treatment, with 50 mg/dL phenol red solution as maker, the gastric empting rates （GER） were identified by its absorbance values, and the intestinal propulsion rats （IPR） were assessed by measuring the ratio of the distance of phenol red running in the intestine and the total lengthC The insulin （INS） and insulin-like receptor （IR） level in gastric antrumwere detected by enzyme-linked immunosorbent assay （ELISA）. Re-sults Compared with the normal control group, the blood glucoses and symptom score in the model group were significantly increased, while GER, IPR and antrum INS and IR decreased （P 〈0.01）. In comparison with the model group, the blood glucoses and symptom score obviously diminished after EA stimulation of acupoint, while the GER, IPR, antrum INS and IR were respectively raised （P 〈0.05, P 〈0.01）. Compared with EA acupoint group, the INS and IR in gastric antrum markedly increased in EA non-acupoint group, while the symptom score reduced （P〈0.05, P〈0.01）. Conclusion EA stimulation can adjust blood glucose level and improve gastrointestinal motility, which may be closely associated with its ef-fects of raising INS and IR in gastric antrum.