漆树木粉抗氧化活性成分的HPLC-MS分析

HPLC-MS Analysis of Antioxidant Components from Rhus verniciflua Stokes Wood Powder

采用有机溶剂对漆树木粉醇提物进行逐级萃取分离,得到石油醚相、乙酸乙酯相、正丁醇相和水相,通过比较4个萃取相对DPPH自由基(DPPH·)、ABTS自由基(ABTS·)和羟基自由基(OH·)的清除能力,探讨了萃取相的抗氧化活性。结果表明:乙酸乙酯相的萃取得率最高,为60.1%;对DPPH·、ABTS·和OH·均具有最强的清除作用,其半数抑制质量浓度(IC50)值分别为19.9、29.74和37.95 mg/L。通过HPLC-MS裂解规律分析,从乙酸乙酯相中鉴定出酚酸类和黄酮类2类化合物,其中酚酸类化合物为:对乙氧基-3-羟基苯甲酸(1)、没食子酸(2)、3,4-二羟基杏仁酸(3)、没食子酸十六烷酯(4)、原儿茶酸(5)和没食子酸乙酯(7);黄酮类化合物为:黄颜木素(6)、3,4',7-三羟基二氢黄酮醇(9)、漆黄素(13)、硫黄菊素(14)、紫铆花素(15)和3,7-二羟基黄酮醇-4'-鼠李糖苷(16)。

The ethanol extract from Rhus verniciflua Stokes wood powder was partitioned with different polarity solvents to obtain petroleum ether fraction, ethyl acetate fraction, n-butylalcohol fraction and water fraction. The DPPH·, ABTS· and OH· scavenging activities of the four fractions were evaluated. The results showed that the extraction yield of ethyl acetate was the highest （60.1%） and its scavenging activities of DPPH·, ABTS· and OH· were the strangest with the IC50 of 19.9, 29.74 and 37.95 mg/L, respectively. Twelve compounds in ethyl acetate fraction were identified by HPLC-MS, including six phenolic acids, i.e., ethoxy-3-hydroxy benzoic acid（1）, gallic acid（2）, 3,4-dihydroxy almond acid（3）, gallic acid cetyl ester（4）, protocatechuic acid（5） and ethyl gallate（7） and six flavonoids, i.e., fustin（6）, 3,4＇,7-trihydroxy-flavanonol（9）, fisetin（13）, sulfuretin（14）, butin（15） and 3,7-dihydroxy flavanone-4＇-rhamnose（16）.