育龄大熊猫阴道源乳酸杆菌的分离鉴定与系统进化

Isolation,identification and phylogenetic analysis of Lactobacillus from vagina of giant panda

旨在对大熊猫阴道源乳酸杆菌进行分离与鉴定。取发情期大熊猫阴道分泌物为试验组,人阴道分泌物为对照组,对其乳酸杆菌进行分离培养,形态学、生化特性、16S r DNA特征鉴定及系统进化分析。结果显示,通过API 50CHL系统鉴定生化特性,结合16S r DNA序列测定和系统进化分析,共分离到6株革兰氏阳性乳酸杆菌,分别为从大熊猫阴道分泌物中分离到的唾液乳酸杆菌GPV01、植物乳酸杆菌GPV02和Lactobacillus saerimneri GPV03,从对照组人阴道分泌物中分离得到嗜酸乳酸杆菌WAV01、卷曲乳酸杆菌WAV02和植物乳酸杆菌WAV03,大熊猫和人阴道中均存在植物乳酸杆菌(分别为GPV02和WAV03)。该结果可为后续研究大熊猫阴道乳酸杆菌生物学特性及开发大熊猫阴道益生菌奠定基础。

In order to separate and identify the Lactobacillus from vagina of giant panda,the vaginal secretions were taken from estrus female giant panda and women. The isolated Lactobacillus strains were identified by morphology,biochemical characteristics,16 S r DNA sequencing and phylogenetic analysis. The results showed that a total of 6gram-positive Lactobacillus strains were obtained. Lactobacillus salivarius GPV01,Lactobacillus plantarum GPV02,Lactobacillus saerimneri GPV03 were isolated from the vaginal secretion of the giant panda（ test group）,and Lactobacillus acidophilus WAV01,Lactobacillus crispatus WAV02,Lactobacillus plantarum WAV03 were isolated from the vaginal secretion of the woman（ control group）. It was interesting that the giant panda and woman all had L. plantarum in the vagina（ GPV02 and WAV03）. This result would lay the foundation for studying biological property of vagina Lactobacillus of giant panda and exploiting vaginal probiotics for giant panda.