摘要
目的研究β-环糊精(β-CD)对共轭亚油酸(CLA)的包合作用。方法利用紫外分光光度法测定CLA和β-CD的结合比、包合常数和其他热力学常数。结果 CLA和β-CD的包合比为1∶1,包合物的吸光度随β-CD浓度的增加而增加,包合形成常数K值随温度的升高而增大,ΔH和ΔS均大于0。结论 CLA与β-CD结合力较强,升温有利于包合反应的进行。β-CD与CLA形成包合物的驱动力是多种作用力共同作用的结果,其中最主要的作用力为疏水作用。
Objective To study the inclusion of conjugated linoleic acid(CLA) by beta cyclodextrin(β-CD).Methods UV spectrophotometry was used to determine the binding ratio,inclusion constant and other thermodynamic constants of CLA and β-CD.Results The combined ratio between beta cyclodextrin and conjugated linoleic acid was 1∶1.The absorbance of inclusion compound increased with the increased concentration of beta cyclodextrin.Inclusion formation constant K value increased with the rise of temperature.ΔH and ΔS are all positive.Conclusion The binding force between CLA and β-CD is strong,and the heating is favorable to the inclusion reaction.
出处
《湖北科技学院学报(医学版)》
2017年第1期1-2,19,共3页
Journal of Hubei University of Science and Technology(Medical Sciences)
基金
2014年度糖尿病心脑血管病变湖北省重点实验室开放专项基金(14zx12)
关键词
Β-环糊精
共轭亚油酸
紫外分光光度法
包合作用
包合形成常数
Beta cyclodextrin
Conjugated linoleic acid
UV spectrometry
Package cooperation with
Inclusion formation constant