紫外分光光度法研究β-环糊精对共轭亚油酸的包合作用

Study on the Inclusion of Conjugated Linoleic Acid by Beta Cyclodextrin on with UV Spectrometry

目的研究β-环糊精(β-CD)对共轭亚油酸(CLA)的包合作用。方法利用紫外分光光度法测定CLA和β-CD的结合比、包合常数和其他热力学常数。结果 CLA和β-CD的包合比为1∶1,包合物的吸光度随β-CD浓度的增加而增加,包合形成常数K值随温度的升高而增大,ΔH和ΔS均大于0。结论 CLA与β-CD结合力较强,升温有利于包合反应的进行。β-CD与CLA形成包合物的驱动力是多种作用力共同作用的结果,其中最主要的作用力为疏水作用。

Objective To study the inclusion of conjugated linoleic acid（CLA） by beta cyclodextrin（β-CD）.Methods UV spectrophotometry was used to determine the binding ratio,inclusion constant and other thermodynamic constants of CLA and β-CD.Results The combined ratio between beta cyclodextrin and conjugated linoleic acid was 1∶1.The absorbance of inclusion compound increased with the increased concentration of beta cyclodextrin.Inclusion formation constant K value increased with the rise of temperature.ΔH and ΔS are all positive.Conclusion The binding force between CLA and β-CD is strong,and the heating is favorable to the inclusion reaction.