响应面法优化紫马铃薯皮花青素的提取工艺

Optimization of Anthocyanins Extraction Conditions from Purple Potato Peel by Response Surface Methodology

利用响应面分析方法研究紫色马铃薯皮花青素得率的最佳提取工艺。采用有机溶剂浸提法,选取提取温度、料液比、浸提时间进行单因素试验的基础上,进行三因素三水平的Central Composite中心组合研究,并运用Design Expect 8.0软件对试验数据进行分析,通过响应面分析法对提取条件进行了优化。结果表明最佳工艺条件为:提取温度60℃、提取时间102 min、料液比1∶34(g/m L)。在此条件下,紫色马铃薯皮花青素得率可达1.13 mg/g。同时研究发现紫色马铃薯皮的花青素含量存在明显的品种间差异,其中品种"黑金刚"的花青素得率最高,达到1.53 mg/g。

The optimum extraction conditions of anthocyanins from purple potato peel by the response surface methodology were studied. On the basis of single factor test,the extracting temperature,solid-liquid ratio and extraction time were selected for the three-factor,three-level Central Composite experimental design. Design Expect 8.0 software was employed to analyze the test data and the response surface method was applied to optimize the extraction conditions. The results indicated the optimum conditions for organic reagents extraction were as follows：the time was 102 min,material-to-liquid ratio was 1 ∶ 34（g/m L）,extracting temperature was 60 ℃.Under these conditions,the maximal yield of anthocyanin reached 1.13 mg/g,which was well matched with the predicted value. Meanwhile,great variation in the yields of anthocyanins was found among different varieties,of which variety‘heijinggang＇had the highest extraction yield of anthocyanins,reaching 1.53 mg/g.