黄连属部分药用植物遗传多样性与亲缘关系的SCoT分析

Analysis on genetic diversity and genetic relationship of medicinal species in Coptis by SCoT

采用SCoT分子标记分析4种黄连属药用植物的遗传多样性和亲缘关系,并用POPGENE计算遗传参数,TREECONW软件进行聚类分析。结果表明:28条引物共检测到434条带,其中多态性条带430条;各遗传参数均表明黄连属药用植物种间的遗传多样性极为丰富(种间PPB=99.1%,N_a=1.990 6,N_e=1.329 3,H=0.212 2,I=0.337 8),但种内遗传多样性较低(种内PPB=16.8%,N_a=1.168 2,N_e=1.073 0,H=0.043 7,I=0.067 7);遗传分化系数G_(st)=0.794 0,说明79.4%的遗传变异来源于种间;同种黄连属药用植物的不同植株均聚在一起,证实SCoT分子标记可作为黄连属药用植物亲缘关系研究的有效方法之一。

The genetic diversity and genetic relationship among four medicinal species of Coptis were detected by the approach of sequence-related amplified polymorphism（ SCoT）. The associated genetic parameters were calculated by POPGENE1. 31. The systematic diagram of genetic relationship were clustered by TREECONW. The results showed that a total of 434 bands were produced by using 28 primers,of which 430 were polymorphic loci. There was a high level of genetic diversity among species（ PPB = 99. 1%,N_a= 1. 990 6,N_e= 1. 329 3,H = 0. 212 2,I = 0. 337 8）. However,genetic diversity was lower within species,the average of genetic parameters was PPB = 16. 8%,N_a=1. 168 2,N_e= 1. 073 0,H = 0. 043 7,I = 0. 067 7. The Nei＇s genetic differentiation coefficient was 0. 794 0,that indicated that most of the genetic variation existed among species. By clustering analysis,different individuals gathered in the same group. The results confirmed that SCoT marker can be used as one of the effective methods to reveal the genetic diversity and relationship among medicinal species of Coptis.