基于指标成分与近红外光谱对宁夏野生和栽培甘草的比较鉴别研究

Comparative identification studies on wild and cultivated Glycyrrhiza uralensis produced Ningxia based on index components and near-infrared spectroscopy

比较宁夏乌拉尔甘草(以下均简称甘草)中基于指标成分含量的色谱鉴别和基于近红外光谱的光谱鉴别差异,建立快速有效鉴别宁夏野生和栽培甘草的方法。采用HPLC和紫外光谱法测定9批野生甘草和14批栽培甘草中甘草苷、甘草酸和总黄酮的含量并采集其近红外光谱信息。结果显示,基于指标成分定量的色谱鉴别无法有效鉴别宁夏野生和栽培甘草,近红外光谱结合聚类分析和主成分分析能够直观、快速、有效鉴别宁夏野生和栽培甘草样品,为甘草生长方式的鉴别及应用研究提供有效方法。同时,为甘草的品种、产地及规格等方面的鉴别提供了研究思路。

This study is to construct a rapid and effective method for identification of wild and cultivated Glycyrrhiza uralensis（ hereinafter referred to as Glycyrrhizae Radix et Rhizoma） from Ningxia by comparison of the difference in chromatography identification based on index components and near-infrared spectroscopy identification. HPLC and UV methods were used to determine the content of liquiritin,glycyrrhizate and total flavonoids for 9 wild Glycyrrhizae Radix et Rhizoma and 14 cultivated Glycyrrhizae Radix et Rhizoma samples,and the near-infrared spectroscopy was also,collected. The results illustrated that the chromatography identification based on index components could not identify wild and cultivated Glycyrrhizae Radix et Rhizoma from Ningxia,while near-infrared spectroscopy could quickly and effectively achieve it. It provides an effective method for the growth pattern identification and application of Glycyrrhizae Radix et Rhizoma.