基于UPLC-Q-TOF-MS技术的芩百清肺浓缩丸血清药物化学初步研究

Serum pharmacochemistry of Qinbai Qingfei concentrated pellets based on UPLC-Q-TOF-MS

应用UPLC-Q-TOF-MS技术和血清药物化学理论分析检测芩百清肺浓缩丸的入血成分。灌胃给予大鼠芩百清肺浓缩丸溶液后,肝门静脉采血,血清经SPE处理,采用ACQUITY UPLC BEH C_(18)色谱柱(2.1 mm×100 mm,1.7μm),0.1%甲酸水(A)-0.1%甲酸乙腈(B)流动相系统进行梯度洗脱,流速0.3 m L·min^(-1),柱温35℃。通过对比分析体外样品、空白血清及含药血清,同时结合Peakview和Metabolitepilot软件鉴定和表征其血中移行成分,在给药后的大鼠血清中分析鉴定了28个血中移行成分,其中18个原型成分,10个代谢产物。应用UPLC-Q-TOF-MS技术和血清药物化学理论较为全面地阐明了芩百清肺浓缩丸在大鼠血清中的移行成分,为进一步研究其体内代谢过程以及药效物质基础提供了科学依据。

To analyze the main components of Qinbai Qingfei concentrated pellets in rat serum with UPLC-Q-TOF-MS technology and serum pharmacochemistry theory. After garage administration with Qinbai Qingfei concentrated pellets, blood was collected from hepatic portal vein. ACQUITY UPLC BEH C18 （2. 1 mm × 100 mm, 1.7 μm） was used, with 0. 1% formic acid agueous solution（ A）- 0. 1% formic acid and acetonitrile（B） as the mobile phase for gradient elution. The flow rate was 0. 3 mL min-1, the column temper- ature was maintained at 35 ~C. Through the comparative analysis fingerprints of Qinbai Qingfei concentrated pellets, drug containing- serum and blank serum, and with the help Peakview and Metabolitepilot software, components in serum were defined. A total of 28 compounds were identified, including 18 prototypes and 10 metabolites. As a result, UPLC-Q-TOF-MS technology and serum pharma- cochemistry theory were applied to comprehensively expound Qinbai Qingfei concentrated pellets＇ constituents migrating to rat serum, and provide scientific basis for further studies for in vivo metabolic process and effective material base.