大鼠低氧性肺血管重塑时硫化氢对Ⅰ、Ⅲ型胶原蛋白在肺血管壁异常堆积的调节作用

Regulatory effects of hydrogen sulfide on abnormal accumulation of typeⅠand Ⅲ collagen in pulmonary vascular wall of rats with hypoxic pulmonary vascular remodeling

目的探讨大鼠低氧性肺血管重塑时硫化氢对Ⅰ、Ⅲ型胶原蛋白在肺血管壁异常堆积的调节作用。方法购买首都医科大学实验动物中心提供的38只健康雄性Wistar大鼠,依据随机数字表法将大鼠分为低氧组(n=14)、低氧+硫氢化钠(Na HS)组(n=12)、对照组(n=12),建立大鼠HPH模型,监测其血流动力学,测定其血浆H2S含量,检测其肺组织中Ⅰ、Ⅲ型胶原蛋白,最后进行结果判定与半定量分析、统计学分析。结果低氧组大鼠的m PAP、RV/(LV+SP)均显著高于低氧+Na HS组、对照组(P<0.05),血浆H2S含量显著低于低氧+Na HS组、对照组(P<0.05),肺小型肌性动脉Ⅰ、Ⅲ型胶原蛋白表达均显著高于低氧+Na HS组、对照组(P<0.05);低氧组、低氧+Na HS组、对照组大鼠肺中型肌性动脉Ⅰ型胶原蛋白表达逐渐降低(P<0.05),低氧组、低氧+Na HS组大鼠肺中型肌性动脉Ⅲ型胶原蛋白表达均显著高于对照组(P<0.05)。低氧组大鼠肺小型肌性动脉、肺中型肌性动脉Ⅰ型胶原m RNA表达均显著高于低氧+Na HS组、对照组(P<0.05);低氧组、对照组、低氧+Na HS组大鼠肺小型肌性动脉、肺中型肌性动脉Ⅲ型胶原m RNA表达均逐渐降低(P<0.05)。结论大鼠低氧性肺血管重塑时硫化氢能够有效抑制Ⅰ、Ⅲ型胶原蛋白及其m RNA在肺血管壁的表达,进而可能对低氧性肺血管重塑进行有效缓解,值得临床充分重视。

Objective To investigate the regulatory effects of hydrogen sulfide on abnormal accumulation of type Ⅰ and Ⅲ collagen in pulmonary vascular wall of rats with hypoxic pulmonary vascular remodeling. Methods A total of 38 eases of healthy male Wistar rats purchased from the experimental animal center of Capital Medical University, these rats were divided into hypoxia group （n=14）, hypoxia＋sodium hydrosulfide （NariS） group （n=12） and control group （n=12） according to the random number table method, rat model of HPH was established, hemodynamics was monitored, contents of H2S in plasma were determined, type Ⅰ and Ⅲ collagen in the lung tissues were detected, finally the results were determined and semi quantitative analyzed and statistically analyzed. Results The mPAP level, RV/（LV＋SP） of the hypoxia group were significantly higher（P〈0.05）, the content of plasma H2S was significantly lower （P〈0.05）, the expression of type Ⅰ and Ⅲ collagen in pulmonary small muscular arteries were significantly higher than the hypoxia＋NaHS group, control group（P〈0.05）; The expressions of type Ⅰ collagen in lung and muscular artery of the hypoxia group, hypoxia＋NaHS group, control group decreased gradually（P〈0.05）, the expressions of type m collagen in pulmonary muscular medium artery of the hypoxia group, hypoxia＋NaHS group were significantly higher than the control group （P〈0.05）. The expressions of type Ⅰ collagen mRNA in pulmonary small muscular arteries, medium-sized pulmonary muscular artery of the hypoxia group were significantly higher than the hypoxia＋NaHS group, control group（P〈0.05）;The expressions of type Ⅲ collagen mRNA in pulmonary small muscular arteries, medium-sized pulmonary muscular arteries of the hypoxia group, hypoxia group＋NaHS group, control group were gradually decreased （P〈0.05）. Conclusion Hydrogen sulfide can effectively inhibit the expressions of type Ⅰ and Ⅲ collagen and mRNA in pulmonary vascular wall, and it may be able to alleviate the pulmonary vascular remodeling of rats, so is worthy of the clinical full attention.