摘要
目的探讨大鼠低氧性肺血管重塑时硫化氢对Ⅰ、Ⅲ型胶原蛋白在肺血管壁异常堆积的调节作用。方法购买首都医科大学实验动物中心提供的38只健康雄性Wistar大鼠,依据随机数字表法将大鼠分为低氧组(n=14)、低氧+硫氢化钠(Na HS)组(n=12)、对照组(n=12),建立大鼠HPH模型,监测其血流动力学,测定其血浆H2S含量,检测其肺组织中Ⅰ、Ⅲ型胶原蛋白,最后进行结果判定与半定量分析、统计学分析。结果低氧组大鼠的m PAP、RV/(LV+SP)均显著高于低氧+Na HS组、对照组(P<0.05),血浆H2S含量显著低于低氧+Na HS组、对照组(P<0.05),肺小型肌性动脉Ⅰ、Ⅲ型胶原蛋白表达均显著高于低氧+Na HS组、对照组(P<0.05);低氧组、低氧+Na HS组、对照组大鼠肺中型肌性动脉Ⅰ型胶原蛋白表达逐渐降低(P<0.05),低氧组、低氧+Na HS组大鼠肺中型肌性动脉Ⅲ型胶原蛋白表达均显著高于对照组(P<0.05)。低氧组大鼠肺小型肌性动脉、肺中型肌性动脉Ⅰ型胶原m RNA表达均显著高于低氧+Na HS组、对照组(P<0.05);低氧组、对照组、低氧+Na HS组大鼠肺小型肌性动脉、肺中型肌性动脉Ⅲ型胶原m RNA表达均逐渐降低(P<0.05)。结论大鼠低氧性肺血管重塑时硫化氢能够有效抑制Ⅰ、Ⅲ型胶原蛋白及其m RNA在肺血管壁的表达,进而可能对低氧性肺血管重塑进行有效缓解,值得临床充分重视。
Objective To investigate the regulatory effects of hydrogen sulfide on abnormal accumulation of type Ⅰ and Ⅲ collagen in pulmonary vascular wall of rats with hypoxic pulmonary vascular remodeling. Methods A total of 38 eases of healthy male Wistar rats purchased from the experimental animal center of Capital Medical University, these rats were divided into hypoxia group (n=14), hypoxia+sodium hydrosulfide (NariS) group (n=12) and control group (n=12) according to the random number table method, rat model of HPH was established, hemodynamics was monitored, contents of H2S in plasma were determined, type Ⅰ and Ⅲ collagen in the lung tissues were detected, finally the results were determined and semi quantitative analyzed and statistically analyzed. Results The mPAP level, RV/(LV+SP) of the hypoxia group were significantly higher(P〈0.05), the content of plasma H2S was significantly lower (P〈0.05), the expression of type Ⅰ and Ⅲ collagen in pulmonary small muscular arteries were significantly higher than the hypoxia+NaHS group, control group(P〈0.05); The expressions of type Ⅰ collagen in lung and muscular artery of the hypoxia group, hypoxia+NaHS group, control group decreased gradually(P〈0.05), the expressions of type m collagen in pulmonary muscular medium artery of the hypoxia group, hypoxia+NaHS group were significantly higher than the control group (P〈0.05). The expressions of type Ⅰ collagen mRNA in pulmonary small muscular arteries, medium-sized pulmonary muscular artery of the hypoxia group were significantly higher than the hypoxia+NaHS group, control group(P〈0.05);The expressions of type Ⅲ collagen mRNA in pulmonary small muscular arteries, medium-sized pulmonary muscular arteries of the hypoxia group, hypoxia group+NaHS group, control group were gradually decreased (P〈0.05). Conclusion Hydrogen sulfide can effectively inhibit the expressions of type Ⅰ and Ⅲ collagen and mRNA in pulmonary vascular wall, and it may be able to alleviate the pulmonary vascular remodeling of rats, so is worthy of the clinical full attention.
出处
《中国现代医生》
2016年第36期37-40,共4页
China Modern Doctor
基金
浙江省温岭市科技计划项目(2006-2009)
关键词
大鼠低氧性肺血管重塑
硫化氢
Ⅰ、Ⅲ型胶原蛋白
肺血管壁异常堆积
调节作用
Hypoxic pulmonary vascular remodeling of rats
Hydrogen sulfide
Collagen type Ⅰ and Ⅲ
Abnormal accumulation of pulmonary vascular wall
Regulation