市售夏枯草饮片质量调查及评价标准研究

Research on quality investigation and evaluation criteria of commercially available Prunellae Spica

为了对夏枯草饮片质量进行全面的评价和控制,对市售18批饮片进行了外观性状考察、TLC鉴别以及水分、灰分、浸出物检测,建立了夏枯草饮片HPLC指纹图谱和咖啡酸、迷迭香酸含量测定方法,并结合主成分分析和聚类分析方法用于对不同批次饮片的模式识别。结果表明,18批饮片外观性状均符合药典标准,但少有符合传统对优品夏枯草外观的要求。TLC图谱均可检出迷迭香酸;水分、总灰分、浸出物含量均符合药典要求;酸不溶性灰分除3批外,其余符合药典要求,但不同批次间有较大的差异;指纹图谱的整体相似度较高,批次间主要色谱峰峰面积和批次间咖啡酸、迷迭香酸的含量有较大差异。通过上述研究,对市售饮片质量有了初步了解,建议提高外观评价标准,增加指纹图谱质量评价指标。

In order to evaluate and control their qualities completely, 18 batches of Prunellae Spica were bought,and their appearances, TLC identification, the inspection of moisture, ash, extract content were carried out. Furthermore, the HPLC-fingerprint of Prunellae Spica, the determination; of caffeic acid and rosmarinic acid content and the pattern identification of different batches by principal analysis and cluster analysis have been established. The results showed that the appearance of each batch accorded with the standards of China Pharmacopoeia, but few of them could reach standard of superior grade product; rosmarinic acid could be detected from all batches by TLC; Moisture, total ash and extract content were in line with the requirements of Pharmacopoeia; Acid insoluble ash of all the batches except 3 of them could comply with the requirement of Pharmacopoeia, but there were rather big difference among various batches; the overall similarity of the fingerprints was high, but the chromatographic peak area and the content of the caffeic acid and rosmarinic acid existed rather big difference between batches. Through the research above, the quality of Prunellae Spica was preliminarily understood, and it is recommended to further improve the appearance evaluation criteria, and add fingerprint as a new quality evaluation index.