高速逆流色谱分离纯化番泻叶中的苷类化合物

Separation and purification of glycosidic compounds from Folium Sennae by high-speed counter-current chromatography

采用高速逆流色谱法从中药番泻叶中快速分离得到4个苷类化合物,溶剂体系为正丁醇-水(9:10,V/V),流速2.0 m L/min,检测波长280 nm,转速860 r/min。从200 mg番泻叶正丁醇萃取物中分离得到10 mg芹菜素-6,8-二-C-葡萄糖苷、9 mg山奈酚-3-O-β-D-龙胆二糖苷、15 mg异鼠李素-3-O-β-D-龙胆二糖苷和5 mg番泻苷B,纯度分别为97.6%、98.9%、98.3%和99.3%。该方法稳定、高效,适合番泻叶中苷类化合物的分离纯化。

Four glycosidic compounds were rapidly separated from the n-butyl alcohol of Folium Sennae by high-speed countercurrent chromatography with the solvent system of n-butanol-water （9：10, V/V）. The flow rate is 2.0 mL/min, the detection wavelength is 280 rim, and the rotate speed rating is 860 r/min. Separated from the n-butyl alcohol extracts （200 mg） of Folium Sennae, their structures were identified as apigenin-6, 8-di-C-glycoside （10 mg）, kaempferol-3-O-β - D gentiobioside （9 mg）, isorhamnetin-3-O-β-D gentiobioside （15 mg）, and sennoside B （5 mg） with the purities were respectively 97.6%, 98.9%, 98.3%, and 99.3%. The results show that this method is simple and efficient, which is suitable for the separation and purification of glycosidic compounds from Folium Sennae.