分子印迹柱净化-UPLC-MS/MS测定果蔬制品中展青霉素

Determination of Patulin in Fruit and Vegetable Products by UPLC-MS/MS Coupled with Molecular Imprinting Column

建立分子印迹亲和柱净化-超高效液相色谱串联质谱检测果蔬制品中展青霉素的方法。样品经2.5%乙酸水提取,分子印迹亲和柱净化,超高效液相色谱串联质谱测定,外标法定量。展青霉素在2.0 ng/mL^50.0 ng/mL范围内线性良好(R2≥0.999 8),方法的检出限为2.0μg/L。在添加水平为5.0和50.0μg/L时,4种果蔬制品回收率为78.4%~92.0%,相对标准偏差低于7.2%。该方法准确、稳定、灵敏,能够满足果蔬制品中展青霉素检测与确证的需要。

A molecular imprinting affinity column coupled with ultra performance liquid chromatography tandem mass spectrometric （UPLC-MS/MS） method for the determination of patulin in fruit and vegetable products. Samples were extracted 2.5%acetic acid water. The crude extract was purified on molecular imprinting affinity column. Sample determined and confirmed by UPLC-MS/MS. Patulin chosen showed good linearity the range of 2.0 ng/mL-50.0 ng/mL（R^2≥0.999 8）. The limit of detection（LOD） was 2.0μg/L. The averge recoveries of patulin in four kinds of fruit and vegetable products at spiked levels of 5.0 and 50.0 μg/L ranged from 78.4%to 92.0%with relative standard deviations（RSDs） smaller than 7.2%. The method is accurate, stable, sensitive and suitable for the determination of patulin in fruit and vegetable products.