脂肪干细胞辅助脂肪颗粒移植在填充面部软组织凹陷中的应用

Application of autologous fat granule mixed with adipose-derived stem cell grafting in facial soft tissue depression

目的探讨应用脂肪干细胞（ADSCs）辅助脂肪颗粒移植填充面部软组织凹陷的临床疗效。方法对36例面部软组织凹陷的患者，于股外侧获取自体脂肪颗粒组织，通过分离、纯化获取ADSCs，并与脂肪颗粒充分混合均匀后，填充面部软组织凹陷，以患者主观满意度以及由两位与本治疗无关的医师根据术前术后照片评估临床疗效。结果36例治疗后随访5～16个月，填充后脂肪吸收率低，面部填充区域外形柔和，形态自然，皮肤质地、毛孔、斑点及皱纹均得到明显改善，效果满意。所有患者均未出现感染、硬结、脂肪液化等并发症。结论应用ADSCs辅助脂肪颗粒移植填充面部软组织凹陷，具有微创、取材方便、经济安全、脂肪成活率高的优势，是一种较为理想的治疗面部软组织凹陷的方法。

Objective To explore the clinical effect of treatment on facial soft tissue depression by autologous fat granule mixed with adipose-derived stem cells. Methods A lof of 36 patients with facial soft tissue depression from September 2011 to March 2013 in this clinic were as the research ob jects. The autologous fat granules were harvested from patients＇ lateral thigh. The autologous fat gran ules mixed with adipose-derived stem cells （ADSCs） which were harvested by separating and cleaning were injected into facial soft tissue depression. The standard was based on the subjective satisfaction of patients and the physicians＇ objective assessment; there were two unrelated doctors to reach an objective assessment of the clinical efficacy based on the comparison of preoperative and postoperative photographs.Results A lof of 36 patients were followed up for 5-16 months, low absorption rate of injected fat and significant improvement in shape filling areas were observed, and skin texture, pores, spots and wrinkles were significantly improved. Conclusions Autologous fat granule mixed with ADSCs in the treatment of facial soft tissue depression is a minimally injuried, easily-handled, cheap and safe procedure. It is an ideal treatment of facial soft tissue depression. All the patients have no complications such as infection, induration and fat liquefaction.