温度对脂肪颗粒及脂肪干细胞活性的影响

Effects of temperature on the bioactivity of fat graft and adipose-derived stem cells situated in fat graft

目的探讨不同保存温度对移植脂肪组织的影响，为临床优化自体脂肪移植技术提供理论依据。方法将从健康女性脂肪抽吸所得脂肪颗粒按不同保存温度分组，对每组脂肪颗粒组织形态学、3-磷酸甘油醛脱氢酶活性、脂肪干细胞（ADsCs）提取效率及增殖能力进行观察。结果4℃保存体外脂肪颗粒24h内可以有效保护其活性，室温（25～28℃）保存体外脂肪颗粒可使其活性降低。体外脂肪颗粒提取干细胞的效率及其增殖能力受保存温度及时间的影响，室温（25～28℃）下保存超过1h的体外脂肪颗粒，ADSCs的提取效率及增殖能力明显下降。结论4℃可以有效保证脂肪组织及其所获得ADSCs的活性，在抽吸脂肪组织的过程及体外脂肪组织转运的过程中必须注意体外脂肪组织的低温保存。

Objective To observe the impacts of different storage temperatures on the activities of adipose tissues and the extraction and proliferation of adipose-derived stem cells （ADSCs） situated in harvested fat. Methods Adipose tissues obtained by liposuctions in healthy females were kept in different temperature; all groups of adipose tissues were studied in context of the histomorphology and activity of GAPD as well as the extraction efficiency and proliferation of ADSCs. Results Adipose tissues cryopreserved at 4 ℃ within 24 hours could retain their activities effectively, but their activities would decrease rapidly if they preserved at room temperature. The efficiency of adipose tissues extracting stem cells and their proliferations were affected by storage temperatures and times. If adipose tis sues were preserved at room temperature for over 1 hour, the extraction efficiency and proliferation of their ADSCs would be lower significantly. Conclusions Adipose tissues cryopreserved at 4 ℃ can effectively ensure their activities and their extraction efficiency and proliferation of ADSCs. It is important to preserve adipose tissues at low temperature after harvesting fat graft.