郁杖丹的提取工艺及其保肝作用研究

Study on Extraction Technology and Liver Protection of Yuzhang Pills

目的确定郁杖丹的最佳提取工艺,研究郁杖丹提取物对CCl_4所致小鼠急、慢性肝损伤的保护作用。方法以川芎中的阿魏酸含量为考察指标,采用L_9(3~4)正交试验,考察乙醇浓度、乙醇用量、提取时间及次数对醇提工艺的影响;以虎杖中的虎杖苷含量为考察指标,采用L_9(3~4)正交试验,考察加水量、提取时间及提取次数对水提工艺的影响;以0.125%CCl_4 10 m L·kg^(-1)腹腔注射制备小鼠急性肝损伤模型,检测血清ALT、AST水平,评价郁杖丹提取物对小鼠急性肝损伤的保护作用;以20%CCl_4 5 m L·kg^(-1)皮下注射,每5天1次,连续8周制备小鼠慢性肝损伤模型,以血清ALT、AST、肝脏病理组织学等为评价指标,评价郁杖丹提取物对小鼠慢性肝损伤的保护作用。结果川芎等最佳醇提工艺:5倍量70%乙醇,提取1次,加热回流1.5 h;虎杖等最佳水提工艺:10倍量水,提取3次,每次1 h。郁杖丹高剂量组能显著降低急性肝损伤小鼠的血清ALT、AST(P<0.05);郁杖丹各剂量组均能降低慢性肝损伤小鼠的血清ALT、AST(P<0.05),减轻小鼠肝脏病理损伤程度。结论确定的郁杖丹最佳提取工艺简便,有效成分提取率高,稳定性好。郁杖丹提取物有对CCl_4引起的小鼠急、慢性肝损伤具有一定的保护作用。

OBJECTIVE To optimize the extraction process of Yuzhang pills and study on its protective effect of acute and chronic liver injury in mice induced by CCl4. METHODS L9（3~4） orthogonal test was used to investigate the ethanol concentration, amount of alcohol, extraction time and times, with the content of offerulic acid as an index. L9（3~4） orthogonal test was used to investigate the amount of water,extraction time and extraction times, with the content of polydatin as an index. 0.125%CCl4（10 m L·kg^-1 intraperitoneal injection）-induced acute liver injury model was established in mice, and the liver protection of Yuzhang pills extract was evaluated by the AST and ALT in serum. The chronic hepatic model was built by hypodermic injection of 20% CC14（5 m L·kg^-1）, 5 d one time for 8 weeks, and the liver protection of Yuzhang pills extract was evaluated by the AST and ALT in serum, pathological changes of liver. RESULTS The best extraction process of Chuanxiong Rhizoma and Curumae Radix were using 5 times of 70% ethanol, extract one time for 1.5 h. The best extraction process of Polygoni Cuspidati Rhizoma Et Radix and other herbs were using 10 times of water, extract 3 times for 1.0 h. The high dose group of Yuzhang pills extract could significantly decrease the level of ALT and AST in acute hepatic mice induced by CCl4（P〈0.05）. All groups of Yuzhang pills extract could significantly decrease the level of ALT and AST in chronic hepatic mice induced by CCl4（P〈0.05）, reduced the liver pathological damage degree. CONCLUSION The optimum extraction process of Yuzhang pills is simple, high extraction yield and stable. Yuzhang pills extract has protective effect on the acute and chronic hepatic injury induced by CCl4 in mice.