云南大理猪戊型肝炎病毒分子流行病学调查

Molecular Epidemiology Survey of Swine Hepatitis E Virus in Dali City of Yunnan Province

目的了解云南大理农村的猪群中戊型肝炎病毒（hepatitis Evirus，HEV）感染情况，为HEV的防控提供理论依据。方法利用逆转录巢式聚合酶链反应（RT-nested PCR）技术，对所采集大理41份猪粪便样品进行HEVORF2基因部分片段扩增，经琼脂糖凝胶电泳后对目的条带进行回收纯化及克隆测序，序列利用DNAStar和MEGA4．0软件进行同源性比较和进化树构建。结果RT-nested PCR方法扩增出348bp目的基因序列，系统进化树显示该病毒属于基因4型h亚型HEV。此次实验共检测41份猪粪便样品，其中23份为阳性，阳性率为56．10％。结论大理猪群存在较高的HEV感染率，存在HEV跨种间感染人的风险,应该加强防控以免HEV爆发流行。

Objective To investigate the prevalence of swine HEV infection in rural areas of Dali City in Yunnan province, where raw pork is traditionally consumed. Methods HEV ORF2 was detected in swine stool samples （n = 41 ） by reverse transcription-nested PCR （RT-nested PCR）. The PCR products were detected by electrophoresis on agarose gel and purified, and then cloned into pMD18-T vector and sequenced. The homology and phylogenetic analysis were performed by DNAStar and MEGA4.0 software, respectively. Results The target gene （348 bp） of HEV was amplified by RT-nested PCR. Phylogenetie analysis showed that these isolated viruses were swine HEV genotype 4 and h subtype. In this study, 23 samples were found to have swine HEV, and the positive rate was 56. 10 % （ 23/41 ） . Conclusion Swine HEV infection was highly prevalent in Dali, which indicated a high risk of zoonotic transmission of HEV from swine to humans. Therefore, strategies should be performed to prevent against HEV infection.