结核感染T细胞检测应用于结核杆菌感染的研究

Research T-SPOT.TB applied to Mycobacterium tuberculosis infection

目的评价结核感染T细胞检测(免疫斑点法)(T-SPOT.TB)的等效性,指导结核病的临床诊治。方法对受试者进行T-SPOT.TB、痰涂片、LDH、ADA、hs-CRP、ESR检测,对结果进行观察、比较、分析。结果结核性疾病组T-SPOT.TB的阳性率均显著高于非结核性疾病组和正常对照组T-SPOT.TB的阳性率,差异有统计学意义(P<0.05);而非结核性疾病组和正常对照组T-SPOT.TB的阳性率差异无统计学意义(P>0.05);结核性疾病组中痰涂片阳性的T-SPOT.TB检测阳性率为85.0%,痰涂片阴性的T-SPOT.TB检测阳性率为86.7%,两者比较差异无统计学意义(P>0.05);结核相关生化指标(LDH、ADA、hs-CRP)和ESR检测在T-SPOT.TB阳性组的表达量均显著高于T-SPOT.TB阴性组的表达量,差异有统计学意义(P<0.05)。结论 T-SPOT.TB为判断是否感染结核的可靠指标。

Objective The results were analyzed to evaluate the meaning of T-SPOT.TB in the clinical diagnosis and treatment of tuberculosis.Methods：All the samples were examined by T-SPOT.TB,sputum smear,LDH,ADA,hsCRP and ESR test.All the testing items were performed strictly in accordance with the Reagent testing instructions.Methods The T-SPOT.TB positive rate of the tuberculous group was significantly higher than that of non-tuberculosis disease group and control group（P〈0.05）;However,the T-SPOT.TB positive rate was no statistically significant between non-tuberculosis group and control group.（P〉0.05）.Positive rate of T-SPOT.TB test was 85.0%for Sputum smear-positive cases,and 86.7% for Sputum smear negative cases,with no statistically significant difference（P〈0.05）.It showed that T-SPOT.TB test in the sputum smear negative TB patients had a high sensitivity.The amount of LDH、ADA、hs-CRP and ESR test in T-SPOT.TB positive group were significantly higher than that in T-SPOT.TB negative groups（P〈0.05）.Results Conclusion T-SPOT.TB is a reliable index for judging whether infected with TB.