华中瘤足蕨配子体发育的研究

Gametophyte Development in Plagiogyria euphlebia

华中瘤足蕨Plagiogyria euphlebia孢子在盛有改良Knop’s的液体培养基中生长,用光学显微镜观察并详细记录其孢子萌发及配子体发育的全过程,结果表明:该孢子萌发的类型为书带蕨型(vittaria-type),原叶体发育的类型为铁线蕨型(adiantum-type)。成熟孢子深棕色,不透明,辐射对称,极面观为钝三角形,赤道面观为圆形,三裂缝,表面较光滑,外壁具颗粒。接种后34d左右萌发,形成2~5个细胞长的丝状体,并生出假根;43d后发育成片状体,65d左右形成幼原叶体,刚形成的幼原叶体不对称,成熟原叶体对称,是心脏形的;原叶体边缘具有毛状体,毛状体由单细胞构成;接种85d左右少数原叶体有性器官出现,精子器由3细胞组成,成熟颈卵器颈部由4列细胞组成。

The spores of the Plagiogyria euphlebia was grown in a liquid medium containing modified Knop,and the whole process of spore germination and gametophyte development were recorded by optical microscope. The results showed that： The spore germination was Vittaria-type, and the gametophyte development was Adiantum-type. Mature spores are nigger brown, opaque and radiation symmetry, obtuse triangle in polar view and circular in equatorial view, trilete, peripheral wall with particles and smooth surface.The spores germinated about 34 days after sowing,forming 2-5 cells long of filaments and bring rhizoid. The prothallial plates formed around 43 d after inoculation. The young prothallium developed about 65 d after sowing which were not symmetry. While the mature prothallus was symmetrical cordate type. Papillary trichomes spread on the margin of the prothallium. These papillary trichomes consisted of single cell.Sexual organs appeared on minority prothallus around 85 d after sowing,consisting of 3 cells.The neck of mature archegonium was composed of 4 rows of cells.