运动经PKC-Nrf2途径调节肝HO-1表达及抗氧化能力的研究

The Regulation Role of Exercise on Heme Oxygenase-1 Expression and Antioxidant Capacity in the Liver by PKC-Nrf2 Pathway

为探讨规律耐力运动通过PKC-Nrf2途径提高大鼠肝血红素加氧酶-1(HO-1)表达及抗氧化能力(T-AOC)的效应,将24只成年雄性SD大鼠随机分为安静对照组(A)、运动组(B)与白屈菜赤碱运动组(C)。B、C组进行8周较大强度的持续耐力跑,但C组在每次运动前进行PKC抑制剂即白屈菜赤碱(CHE)处理。实验中观察各组肝细胞膜PKC及肝细胞总PKC的活性水平,Western Blotting观察肝细胞核Nrf2及肝细胞HO-1的蛋白表达水平,以及观察肝细胞HO-1活性和T-AOC水平,结果发现:与A组相比,B组肝细胞膜PKC及肝细胞总PKC的活性显著升高、细胞膜PKC活性/肝细胞总PKC活性的值显著升高(P<0.05),细胞核中的Nrf2蛋白含量(核转位)显著增加(P<0.05),肝细胞HO-1的蛋白表达水平、活性水平及肝细胞T-AOC等均显著升高(P<0.05);耐力运动引起B组中的上述变化在C组中被明显遏制。综上可见,较大强度的规律耐力运动可经过PKC-Nrf2途径,诱导肝细胞HO-1蛋白表达而提高肝脏抗氧化能力。

To explore the effect of regular endurance exercise on Heme Oxygenase-1 （HO-1） expression and total antioxidant capacity （T-AOC） by the PKC-Nrf2 pathway in liver of rats,24 adult male SD rats were randomly divided into quiet control group （A）, exercise group （B）, and CHE and exercise group （C）. Rats in group B and C were interfered by 8 weeks of vigorous intensity endurance running, but group C were treated by chelerythrine （CHE） of PKC inhibitors before each exercise. To observe the activity level of cells membrane PKC,cells total PKC in liver, to observe the expression level of nucleus Nrf2 protein and cells total HO-1 protein in liver by Blotting Western,and to observe the level of HO-1 activity and T-AOC in the liver cells.The results show that the activity of liver cells membrane PKC,cells total PKC activity and the ratio of the two were significantly higher in group B than that in the group A （P〈0.05）,and the expression level of nucleus Nrf2 protein（nuclear translocation） in liver was significantly increased in group B than that in the group A （P 〈0.05）, and the expression and activity level of cells HO-1 protein in liver was significantly increased in group B than that in the group A （P〈 0.05）, and liver cells T-AOC was significantly increased in group B than that in the group A（P 〈0.05）. The changes caused by endurance exercise in group B were significantly curbed in group C. It draw a conclusion that regular vigorous intensity endurance exercise can raise protein expression of HO-1 and T-AOC in rats liver cells by the PKC-Nrf2 pathway.