海南砂仁脂溶性成分的GC-MS分析

GC-MS Analysis of Lipid Soluble Composition from Fruits of Amomum longiligulare

目的:研究海南砂仁的脂溶性成分。方法:利用溶剂分步萃取,得到各萃取部位;采用气相色谱-质谱(GC-MS)联用技术对其石油醚部位的成分进行测定,并与标准图谱对照,确定化合物成分,经色谱峰面积归一化法确定各成分的相对百分含量。结果:从海南砂仁石油醚萃取部位中共分离出76个组分,鉴定了其中的56种成分,所鉴定的成分占总脂溶性成分的68.81%,其中相对质量分数较高的成分有对苯二酚(7.74%)、Z,E-2,13-十八碳二烯-1-醇(6.86%)、E,E-2,13-十八碳二烯-1-醇(5.44%)、4-十一烷基苯酚(3.71%)、(Z)-9-十八碳烯醛(3.65%)、顺式-异油酸(3.39%)及(E,E)-大牻牛儿-3,7(11),9-三烯-6-酮(3.05%)等。结论:本研究首次对海南砂仁的石油醚部位的化学成分进行了分析,该研究为海南砂仁的进一步开发和利用提供了基础研究数据。

Objective：To investigate the chemical constituents in the lipid soluble composition from the fruits of Amomum longiligulare.Methods：The petroleum ether fraction was extracted by solvent step by step and its chemical constituents were separated,determined and identified by gas chromatography-mass spectrometry combined with computer retrieval in compound databases.The relative percentage content of each compound was calculated by peak area normalization method.Results：76 compounds were separated and 56 of them were identified,which covered 68.81% of the total lipid soluble composition.The major constituents with higher relative content were hydroquinone（7.74%）,Z,E-2,13-octadecadien-1-ol（6.86%）,E,E-2,13-octadecadien-1-ol（5.44%）,4-undecylphenol（3.71%）,（Z）-9-octadecenal（3.65%）,cis-vaccenic acid（3.39%）and（E,E）-germacra-3,7（11）,9-trien-6-one（3.05%）.Conclusion：Chemical constituents of the petroleum ether fraction from the fruits of A.longiligulare were analyzed for the first time.The present study provides a chemical basis for A.longiligulare .